The synthesis of kojic acid derivative (KAD) from kojic and palmitic acid (C16:0) in the presence of immobilized lipase from Rhizomucor miehei (commercially known as Lipozyme RMIM), was studied using a shake flask system. Kojic acid is a polyfunctional heterocycles that acts as a source of nucleophile in this reaction allowing the formation of a lipophilic KAD. In this study, the source of bioc...

Abstract In this study, the effect of biosurfactant sophorolipids (SLs) on Rhizomucor miehei lipase (RML) fermentation by Aspergillus oryzae was investigated. With exogenous addition 0.3% (w/v) SLs in initial medium, RML activity reached 430.0 U/mL, an increase 25.0% compared to control group. Subsequently, physiological metabolic responses A. were further explored. The results showed that thou...

In the current study, thermo-tolerant fungal isolates were screened for their ability to metabolize L-methionine by γ-lyase (MGL). Among 63 isolates, fifteen exhibited MGL induction as evidenced qualitative assay method using phenol red. Then, quantitative indicates that coded 26 & 37 most potent production. Fungal mycelium treatment under sonication process proved isolate No. could be release ...

BACKGROUND β-Mannanase randomly cleaves the β-1,4-linked mannan backbone of hemicellulose, which plays the most important role in the enzymatic degradation of mannan. Although the industrial applications of β-mannanase have tremendously expanded in recent years, the wild-type β-mannanases are still defective for some industries. The glycoside hydrolase (GH) family 5 β-mannanase (RmMan5A) from R...