نتایج جستجو برای: recombinant plasmids

تعداد نتایج: 124414  

Journal: :iranian journal of parasitology 0
razi naserifar department of medical parasitology, faculty of medicine, ilam university of medical sciences, ilam, iran fatemeh ghaffarifar department of parasitology and entomology, faculty of medical sciences, tarbiat modares university, tehran, iran abdolhosein dalimi department of parasitology and entomology, faculty of medical sciences, tarbiat modares university, tehran, iran zohreh shar¬ifi blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran kavous solhjoo department of medical microbiology, faculty of medicine, jahrom university of medical sciences, jahrom, iran kami hosseinian khosroshahi department of parasitology and entomology, faculty of medical sciences, tarbiat modares university, tehran, iran

background: severe and fatal complications of toxoplasmosis urge development of effective vaccines against the disease. the current study was performed to evalu­ate cocktail dna vaccine containing plasmids encoding gra5, sag1, and rop2 genes of toxoplasma gondii in balb/c mice in tarbiat modares university in 2012. methods: the plasmids containing complete gra5, sag1, and rop2 genes were mass e...

A.A ZIAEE, ALI A FARHOUDI-MOGHADDAM, NICKOLAY N DOMANSKY,

The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 a...

Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...

We studied the expression of human growth hormone (hGH) in E. coli under a bacteriophage T5-base promoter in a pQE30 expression vector. For an efficient expression of hGH cDNA, a number of codons at the hGH N-terminal coding region were altered based on the E. coli major codons. An over-expression of hGH in the bacteria, carrying the recombinant plasmids, was observed at 37°C in the presence of...

Journal: :applied biotechnology reports 0
fathollah ahmadpour bagher yakhchali seyed safa ali fatemi ali asghar karkhane samira talebi

lipases are versatile biocatalysts with a wide range of application in food, dairy, leather, paper, pharmaceutical and detergent industries.  in this study, mesophilic lipase gene from an indigenous bacillus pumilus f3 that already its gene had been sequenced and  identified, was cloned and expressed in methylotrophic yeast pichia pastoris and codon translation of lipase gene was evaluated in p...

Journal: :iranian journal of biotechnology 2015
xueqin rao jie sun

background: watermelon silver mottle virus (wsmov), which belongs to the genus tospovirus, causes significant loss in cucurbitaceae plants. objectives: development of a highly sensitive and reliable detection method for wsmov.materials and methods: recombinant plasmids for targeting the sequence of nucleocapsid protein gene of wsmov were constructed. sybr green i real-time pcr was established a...

Journal: :Journal of bacteriology 1976
T Horodniceanu D H Bouanchaud

Recombinant plasmids between an R plasmid of the FI group (R162/3) and the sex factor F or HfrH were produced after the conjugal transfer of this R plasmid into HfrH. Three types of recombinant plasmids were identified after the mating of HfrH (R162/3) with recA and rec+ recipients. One specimen of each type (pIP218, pIP222, pIP226) was studied in this report. All three recombinant plasmids car...

H. Mirzahoseini

Chaperones are produced by prokaryotic, yeast and higher eukaryotic cells for various purposes. Over-expression of each chaperone or sets of them affect the production level of a recombinant protein in the cell. On the basis of this hypothesis, five different plasmids with 5 different combinations of 6 chaperones molecule, transformed into Escherichia coli along with human basic Fibroblast Grow...

Journal: :journal of sciences islamic republic of iran 0

we studied the expression of human growth hormone (hgh) in e. coli under a bacteriophage t5-base promoter in a pqe30 expression vector. for an efficient expression of hgh cdna, a number of codons at the hgh n-terminal coding region were altered based on the e. coli major codons. an over-expression of hgh in the bacteria, carrying the recombinant plasmids, was observed at 37°c in the presence of...

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