Proteinase K cleaves a small peptide from native muscle-specific creatine kinase. We present evidence, from the binding of two monoclonal antibodies to formic acidcleavage fragments and proteinase K-digest fragments of chick muscle creatine kinase, that the proteinase K-cleavage site is in the C-terminal region of the molecule. This specificity of proteinase K, which is not normally a highly sp...

Proteinase K is widely used in methods for detection of transcripts in biological specimens by in situ hybridization (ISH). However, treatment with proteinase K hampers detection of RNA and protein simultaneously. We have developed a method for double staining of transcripts and proteins by ISH and IHC staining in imaginal discs and embryos of Drosophila. Instead of treatment with proteinase K,...

The kinetics of slow onset inhibition of Proteinase K by a proteinaceous alkaline protease inhibitor (API) from a Streptomyces sp. is presented. The kinetic analysis revealed competitive inhibition of Proteinase K by API with an IC50 value 5.5 +/- 0.5 x 10-5 m. The progress curves were time-dependent, consistent with a two-step slow tight binding inhibition. The first step involved a rapid equi...

The digestion of ribonuclease A by proteinase K yielded one major degradation product only, which could not be distinguished from ribonuclease S by electrophoretical and immunological methods. This component (ribonuclease K) possessing full catalytic activity was characterized to be (1--20/21--124) ribonuclease A. Combined action of proteinase K and trypsin on ribonuclease A leads to a signific...

We and others have occasionally experienced difficulty in cloning PCR derived DNA fragments (1). This has occurred even after extraction of the DNA with phenol and phenol:chloroform, followed by precipitation with ethanol and/or column purification of the bands. To test the hypothesis that the Taq polymerase remains bound to the DNA and therefore inhibits restriction endonuclease activity, we i...

materials and methods s. sonnei and s .flexneri was identified by common microbiological and serological methods. antibiogram with 18 antibiotics were tested for 34 positive cultures by disc diffusion method. the samples showed considerable resistance to antibiotics. antimicrobial effects of cfcs were tested against s. sonnei and s. flexneri by agar-well assay and broth micro dilution methods. ...

Activation of the Hageman factor-kallikrein-kinin system by serratial 56-kDa proteinase was previously demonstrated (Matsumoto, K., Yamamoto, T., Kamata, T., and Maeda, H. (1984) J. Biochem. (Tokyo) 96, 739-749; Kamata, R., Yamamoto, T., Matsumoto, K., and Maeda, H. (1985) Infect. Immun. 48, 747-753). To investigate whether the activation of the system is specific for 56-kDa proteinase or is fo...

A simple method for DNA purification from various cell types has recently been published (1). In this protocol, cells are just dispersed in guanidine hydrochloride and DNA ethanol precipitated. Unfortunately, in my hands, the DNA was extremely difficult to resuspend when blood was used as a source. Proteinase K has been used in nucleic acid isolation for more than 15 years (2,3) and is known to...