CYP21-4 is a novel Golgi-localized cyclophilin protein involved in oxidative stress tolerance. Here, we generated transgenic plants overexpressing AtCYP21-4 and OsCYP21-4 in potato and rice, respectively. The stems and roots of AtCYP21-4-overexpressing potato plants were longer than those of wild-type (WT) plants, which resulted in heavier tubers. In vitro tuberization in the transgenic potato ...

The Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), is the most destructive insect pest of potato, Solanum tuberosum (L.), in North America. Avidin sequesters available biotin, thereby causing abnormal growth and development of insects. We expressed avidin in two potato lines: MSE149-5Y, a susceptible potato line, and ND5873-15, a line with S. chacoense-deri...

A potato (Solanum tuberosum) cDNA encoding an isoform of disproportionating enzyme (stDPE2) was identified in a functional screen in Escherichia coli. The stDPE2 protein was demonstrated to be present in chloroplasts and to accumulate at times of active starch degradation in potato leaves and tubers. Transgenic potato plants were made in which its presence was almost completely eliminated. It c...

This study established a new system for potato transformation using toxoflavin as selection agent and toxoflavin lyase (tflA) as selectable marker gene. Potato plants expressing tflA was successfully transformed on toxoflavin medium with 27% efficiency, similar to that for the hygromycin/hpt selection system. The transgenic potato expressing tflA also showed resistance to Burkholderia glumea in...

Potato ( Solanum tuberosum L.) cultivar IPB CP1 is suitable as a raw material for the potato chip industry. plants are sensitive to various abiotic stresses such drought, aluminium and salinity, which induce reactive oxygen species (ROS). ROS very toxic plant cells. Superoxide dismutase (SOD) one of enzymes that catalyse H2O2 O2. This study aimed establish transgenic cv. containing MmCuZn‐SOD g...

The effect of Bt-cry1Ia1 (cryV now designated cry1Ia1 under revised nomenclature) of potato transgenic plants on the two species of potato tuber moth, Phthorimaea operculella (Zeller) and Symmetrischema tangolias (Gyen), was tested in Peru. Detached leaf bioassays were done using 10 neonate larvae per replication on each transgenic line of bred potato varieties Atlantic and Spunta. Mortality in...

Transgenic potato and tobacco plants carrying the osmotin gene under the control of the cauliflower mosaic virus 35S promoter constitutively overexpressed osmotin to a level of approximately 2% of total cellular protein. Leaves of transgenic potato plants exhibited delayed development of disease symptoms after inoculation with spore suspensions of Phytophthora infestans, which is the cause of l...

References 1 Greiner, S. et al. (1999) Ectopic expression of a tobacco invertase inhibitor homolog prevents cold-induced sweetening of potato tubers. Nat. Biotechnol. 17, 708–711 2 Hemberg, T. (1985) Potato rest. In Potato Physiology (Li, P.H., ed.), pp. 353–388, Academic Press 3 Suttle, J.C. (1995) Postharvest changes in endogenous ABA levels and ABA metabolism in relation to dormancy in potat...

IbMYB1, a transcription factor (TF) for R2R3-type MYB TFs, is a key regulator of anthocyanin biosynthesis during storage of sweet potatoes. Anthocyanins provide important antioxidants of nutritional value to humans, and also protect plants from oxidative stress. This study aimed to increase transgenic potatoes' (Solanum tuberosum cv. LongShu No.3) tolerance to environmental stress and enhance t...

Cultivated potato is a drought-, salinity-, and frost-sensitive species. The transgenic approach is one of the methods used to mitigate abiotic stress. The utility of transgenic potatoes that have abiotic stress tolerance should be judged from their yield under stress conditions. In order to establish transgenic potato lines with the AtDREB1A gene that could be used in practical applications, w...