Polynucleotide kinase from E. coli infected with the PseT 1 mutant of bacteriophage T4 has been isolated. The PseT 1 enzyme purifies similarly to normal polynucleotide kinase and effectively transfers the gamma phosphate of ATP to the 5' terminal hydroxyl of DNA and RNA. The PseT 1 and normal enzymes require similar magnesium ion concentrations, have the same pH optima and are both inhibited by...

CsgD and cyclic-3',5'-di-guanylate are key regulators of biofilm formation in Salmonella enterica serovar Typhimurium. Our results show that polynucleotide phosphorylase and NlpI oppositely altered expression of CsgD. Polynucleotide phosphorylase and NlpI also had opposite effects on the expression of yjcC, which codes for a cyclic-3',5'-di-guanylate phosphodiesterase affecting CsgD expression.

The gene for the enzyme guanosine pentaphosphate synthetase I (GPSI) from Streptomyces antibioticus has been cloned and sequenced. The cloned gene functioned as a template in the streptomycete coupled transcription-translation system and directed the synthesis of a protein with the properties expected for GPSI. Sequencing of the cloned gene identified an open reading frame of 740 amino acids wh...

cupper oxide structures with a variety of novel morphologies are synthesized using cupper foil as substrate via a solution route. the structure, morphology and phase of the as-synthesized nanostructures are analyzed by various techniques. sem images show gradual development of hierarchical structures of copper oxide with different morphology. in order to study the effect of reaction time and te...

in this pepper fe2o3 nanoparticles were synthesized via a fast microwave method. then fe2o3-tio2 nanocomposites were synthesized by a sonochemical-assisted method. the prepared products were characterized by x-ray diffraction pattern, scanning electron microscopy and fourier transform infrared spectroscopy. the photocatalytic behaviour of fe2o3-tio2 nanocomposites was evaluated using the degrad...

The mechanism of action of T4-induced polynu-cleotide kinase on short single-stranded deoxyoligonucleotideshas been investigated.It was found that the phosphorylationreaction catalyzed by T, polynucleotide kinase could be re-versed. Thus, a 5 ’-3*P-labeleddeoxyoligonucleotide and ADPon incubation with the enzyme formed [Y-~~PJATP.In addi-tion some radioac...

Two structurally similar bis-phenanthridinium–adenine conjugates (equipped with one or two adenines, respectively), exhibiting strong ds-DNA/RNA groove binding, revealed ratiometric fluorescent recognition of alternating AT-DNA with respect to other ds-DNA/RNA and ss-RNA. Further, CD spectra pattern of adenine–bis-phenanthridinium conjugate/polynucleotide complexes strongly depended on polynucl...

In this work, we consider the motion of a polaron in polynucleotide Holstein molecular chain constant electric field. It is shown that character depends not only on chosen parameters chain, but also initial distribution charge along chain. for small set value field intensity and fixed values parameters, changing it possible to observe either uniform movement or an oscillatory mode movement.

Mice hyperimmunized with polyinosinic . polycytidylic acid complexed with methylated bovine serum albumin were protected against challenge with Friend leukemia virus. The virus was inoculated at the time when anti bodies reacting to the polynucleotide structure had reached an appreciable level. A sparing effect of approximately 1.5 logs in infectivity was afforded the immunized mice. Our result...