Several in vitro phototoxicity methods have been developed to assess the phototoxic potential of substances. These can be classified into two groups: the methods for screening purposes and tests focusing on the specific mechanisms of phototoxic reactions. Among these methods the 3T3 Neutral Red uptake phototoxicity test (3T3 NRU PT) was accepted as an established alternative method by ECVAM. In...

Fluoroquinolones (FQs) are extensively used in bacterial keratitis and other intraocular infections. Since eye is constantly exposed to light, incidence of ocular phototoxicity due to commonly used FQs is of great interest for their safe use. Phototoxicity of commonly used FQs (ciprofloxacin, lomefloxacin, pefloxacin, ofloxacin, sparfloxacin and gatifloxacin), has been evaluated by using HET-CA...

Objective: Phototoxicity is a kind of dermatitis that is activated by exposure to ultraviolet light following the administration of some drugs or natural products. Artemia salina (A. salina) (brine shrimp) has been effectively applied for toxicity testing and is perfect for biological screening of many chemicals for simultaneous evaluation of toxicity and phototoxicity. The objective of this st...

objective: phototoxicity is a kind of dermatitis that is activated by exposure to ultraviolet light following the administration of some drugs or natural products. artemia salina (a. salina) (brine shrimp) has been effectively applied for toxicity testing and is perfect for biological screening of many chemicals for simultaneous evaluation of toxicity and phototoxicity. the objective of this st...

The spectroscopic observation of 1268-nm emission of singlet oxygen photosensitized by tetracyclines in oxygenated solutions at room temperature is reported. In the series demeclocycline, tetracycline, and minocycline, the efficiency of singlet oxygen generation is found to parallel the clinical observation of relative frequency of phototoxicity of these antibiotics, suggesting singlet oxygen g...

Phototoxic properties of systemically applied pharmaceuticals may be the cause of serious adverse drug reactions. Therefore, a reliable preclinical photosafety assessment strategy, combining in vitro and in vivo approaches in a quantitative manner, is important and has not been described so far. Here, we report the establishment of an optimized modified murine local lymph node assay (LLNA), ada...

Rational, computationally guided, molecular design affords push–pull BODIPY photosensitizers with balanced brightness and phototoxicity for image-guided photodynamic therapy.

Background Material and Methods Results Conclusions.