Background: Human epidermal growth factor receptor 2 (HER2) is over-expressed in breast, ovarian, gastric, and prostate cancers used as a tumor marker the diagnosis of cancer. Monoclonal antibodies have been diagnostic therapeutic tool against HER2. Because difficulties associated with stability complexity construct high cost antibody production, we aimed to investigate, cloning, expression HER...

The low distribution of hydrophobic anticancer drugs in patients is one the biggest limitations during conventional chemotherapy. SDS-based polyelectrolyte multicore nanocarriers (NCs) prepared according to layer by (LbL) procedure can release paclitaxel (PTX), and selectively kill cancer cells. Our main objective was verify antitumor properties PTX-loaded NCs examine whether drug encapsulated ...

Background: Interferon-gamma [IFN-γ) is the most important cytokine in immune system. This protein has been expressed bacterial cells. However, cloning not an easy task. We aimed to clone, express, and purify recombinant mouse IFN-γ overcome problems favor of commercial purposes. Materials Methods: To amplify gene product for cloning, we primarily designed two specific primers target gene. Foll...

Abstract The current work aims to evaluate the growth rate, molecular patterns and biological activities of Arthrospira platensis cultivated in culture enriched with different chelated minerals (Fe, Zn, Cu, Mn, Mg) leather protein hydrolysates (LPHs) glycine addition determine phytochemical contents modified Zarrouk medium. Also, study profile variations (using SDS-PAGE, Comet assay, SCOT-PCR) ...

Salt concentration governs nucleic acid hybridization according to the Schildkraut-Lifson equation. High concentrations of SDS are used in some common protocols, but the effects of SDS on hybridization stringency have not been reported. We investigated hybridization parameters in solutions containing SDS. With targets immobilized on nylon membranes and PCR- or transcription-generated probes, we...

Rabies is an infectious viral disease without any cure. Autophagy considered as a method for reducing the damage to cell and its organelles. In some virus-infected cells, autophagy plays antiviral effect, while it acts in opposite manner other cells. Beclin1 (Atg6/Vps30) main regulator of autophagy. The present study aims build recombinant beclin1 stimulating evaluating effects mice infected wi...

Staphylococcus aureus reacts to changing environmental conditions such as heat, pH, and chemicals through global regulators such as the sae (S. aureus exoprotein expression) two-component signaling system. Subinhibitory concentrations of some antibiotics were shown to increase virulence factor expression. Here, we investigated the S. aureus stress response to sublethal concentrations of a commo...