JCB • VOLUME 169 • NUMBER 1 • 2005 16 Folding in a crowd roteins fold better in a crowd, as predicted by a biophysical model from Margaret Cheung, Dmitri Klimov, and D. Thirumalai (University of Maryland, College Park, MD). Interactions between a protein and large nearby molecules produce repulsive forces at distances that can be as large as the protein itself. The group modeled the energetic e...

JCB • VOLUME 169 • NUMBER 1 • 2005 16 Folding in a crowd roteins fold better in a crowd, as predicted by a biophysical model from Margaret Cheung, Dmitri Klimov, and D. Thirumalai (University of Maryland, College Park, MD). Interactions between a protein and large nearby molecules produce repulsive forces at distances that can be as large as the protein itself. The group modeled the energetic e...

diopathic dilated cardiomypathy (DCM) is characterized y the progressive depression of cardiac function and left entricular dilation in the absence of coronary artery disease. here is evidence to suggest that humoral immunity plays n important role in the pathogenesis of DCM. In particlar, the role of autoantibodies in the pathogenesis of DCM as been emphasized (1). Sera from patients with DCM ...

l ycosylation is one of the most important types of posttranslational modification (PTM) in proteins. Due to the high d e g ree of hetero g e n e i t y, the characterization of glycans is a challenging task. Mass spectro m e t ry (MS) is a primary tool for biopolymer analysis; howe ve r, the characterization of (native) glycans is complicated by the time-consuming sample preparation and their p...

HE MAJOR METHODS CURRENTLY IN USE for the determination of uric cid in biologic fluids are the colorimetric (1-3), the titrimetric (4), and he enzymatic (5-7). All of these methods are performed directly on the luid to be measured and are therefore subject to the effects of interfering ubstances present in these fluids. Since the colorimetric and titrimetric echnics depend on the reducing abili...

The expression of transgenes encoding proteins modified o contain residues that impart a particular property, ‘tagged roteins’, is central to the post-genomic analysis of any organsm. Trypanosoma brucei is a model kinetoplastid protozoan athogen and has the most advanced repertoire of tools for everse genetic analysis available for any protozoan. The vast ajority of these tools take advantage o...

cell or directly of the antibody binding capacity using standard 2 ince the 1980s, multiparametric flow cytometry (FCM) was rought to the clinical laboratory for diagnostic immunopheotyping. The expression of antibodies bound to the embrane or intracellular receptors has been generally efined as positive or negative with a cutoff set relative to a onstaining control population. It is known that...

In this study, the relationship among four species of Polygonum (including P. hyrcanicum (three samples), P. persicaria, P. avicular, and P. hydropiper) was investigated by GC profiling. Furthermore, the major compounds of the ethylic ether extract of P. hyrcanicum were identified by GC/MS as: α-bisabolol (17.5%), cedrol (15.9%), sesquisabinene hydra...

Targeting the inner nuclear membrane roteins destined for the inner nuclear membrane (INM) start out in the peripheral ER. Diffusion from the ER will get them to the contiguous outer nuclear membrane (ONM), but the next step could involve either vesicular transport, short-lived fusions between INM and ONM, or movement along the lipid bilayers surrounding nuclear pores. On page 1051, Ohba et al....