Dehydrated Mueller-Hinton agar from five different manufacturers was used to prepare plates containing 4% NaCl and 6 micrograms of oxacillin per ml to screen for oxacillin resistance in Staphylococcus aureus. A total of 137 isolates which included 109 oxacillin-resistant isolates obtained from at least eight geographic areas were examined. Results were compared to MICs obtained by using a stand...

UNLABELLED Cefoxitin is a potent inducer of the mecA regulatory system. It is being recommended for detection of methicillin resistance in Staphylococcus aureus (MRSA) when using disk diffusion testing. The aim of our study was to evaluate the efficacy of cefoxitin disc diffusion test to characterize MRSA and compare it with oxacillin agar screening and detection of mecA gene by PCR. MATERIAL...

  BackgroundandObjectives:RapidandaccuratedetectionofmethicillinresistantStaphylococcus aureus (MRSA) is an important role of clinical microbiology laboratories to avoid treatment failure. The aim of this study was to compare conventional methods against the E-test minimum inhibitory concentration (MIC) method to determine the best phenotypic method. Materials a...

backgroundandobjectives:rapidandaccuratedetectionofmethicillinresistantstaphylococcus aureus (mrsa) is an important role of clinical microbiology laboratories to avoid treatment failure. the aim of this study was to compare conventional methods against the e-test minimum inhibitory concentration (mic) method to determine the best phenotypic method. materials and methods: methicillin resistance...

background: methicillin resistant staphylococcus aureus (mrsa) is one of the major agents for increasing number of serious hospital and community acquired infections. the aim of this study was to investigate the occurrence of the mrsa and meca gene among nosocomial and environmental specimens in kurdistan hospitals and determining the antibiotic resistance of the isolates. materials and methods...

The emergence of Methicillin-resistant Staphylococcus aureus (MRSA) has posed a serious therapeutic challenge. We report the prevalence and antibiotic susceptibility pattern of MRSA in the hospitalized patients in a rural tertiary care hospital in India. The study comprised of 97 Staphylococcus aureus isolated from a total of 400 clinical samples obtained from hospitalized patients. Antibiotic ...

A multilaboratory study was undertaken to determine the accuracy of the current National Committee for Clinical Laboratory Standards (NCCLS) oxacillin breakpoints for broth microdilution and disk diffusion testing of coagulase-negative staphylococci (CoNS) by using a PCR assay for mecA as the reference method. Fifty well-characterized strains of CoNS were tested for oxacillin susceptibility by ...

The heterogeneous expression of methicillin resistance in Staphylococcus aureus affects the efficiency of tests available to detect it. Not all laboratories have access to accurate molecular tests used for this purpose. This study compares the performances of four phenotypic tests used to detect methicillin resistant S. aureus (MRSA) with the mecA gene polymerase chain reaction. Two hundred thi...

Introduction The heterogeneous expression of methicillin resistance in Staphylococcus aureus (MRSA) affects the efficiency of tests available to detect it. The objective of this study was to assess four phenotypic tests used to detect MRSA. Methods This is an analytical comparative study conducted among sudanese patients during period from May 2012 to July 2014, Staphylococcus aureus strains ...

Background and Aims: The aim of present study was to investigate the molecular characteristic of Staphylococcus aureus to detect mec A gene and to type SCCmec in strain isolated from healthy worker at Larestan Hospital. Material and Methods: This study was carried out from the 250 nasal swab healthy worker  at Larestan hospital. Multiplex polymerase chain reaction for mecA gene was perf...