One of the most important QC tests of whole-cell pertussis vaccine (WCPV) is potency test. In this regard, mouse protection test (MPT) is the current potency method, which is associated with severe animal distress and large variability in results. The purpose of this study was to assess Pertussis Serological Potency Test (PSPT) as a serological alternative method to intracerebral challenge in M...

An enzymatic assay was developed to measure tetrahydromethanopterin (H(4)MPT) levels in wild-type and mutant cells of Methylobacterium extorquens AM1. H(4)MPT was detectable in wild-type cells but not in strains with a mutation of either the orf4 or the dmrA gene, suggesting a role for these two genes in H(4)MPT biosynthesis. The protein encoded by orf4 catalyzed the reaction of ribofuranosylam...

Multinomial processing tree (MPT) models are a class of measurement models that account for categorical data by assuming a finite number of underlying cognitive processes. Traditionally, data are aggregated across participants and analyzed under the assumption of independently and identically distributed observations. Hierarchical Bayesian extensions of MPT models explicitly account for partici...

The methylotrophic proteobacterium Methylobacterium extorquens AM1 possesses tetrahydromethanopterin (H(4)MPT)-dependent enzymes, which are otherwise specific to methanogenic and sulfate-reducing archaea and which have been suggested to be involved in formaldehyde oxidation to CO(2) in M. extorquens AM1. The distribution of H(4)MPT-dependent enzyme activities in cell extracts of methylotrophic ...

An early event in the induction of apoptosis is cytochrome c (Cyt c) release from mitochondria. We investigated the involvement of mitochondrial permeability transition (MPT) and mitochondrial swelling in Cyt c release from mitochondria treated with alloxan and/or calcium (Ca). When mitochondria were treated with a high concentration of Ca alone or Ca with alloxan (alloxan-Ca), the MPT was acco...

An improved maximum power tracking (MPT) approach with dither signal injection is presented. It incorporates a “shared-bus’’ current-sharing method that can regulate many paralleled current-mode dc-dc converters. Through simulation, the approach yields -nearly uniform currentsharing as well as reliable MPT performance. Utilizing the combined current-sharing and MPT approaches, three proposed ar...

Molybdenum cofactor (Moco) biosynthesis is linked to c-Jun N-terminal kinase (JNK) signaling in Drosophila through MoaE, a molybdopterin (MPT) synthase subunit that is also a component of the Ada Two A containing (ATAC) acetyltransferase complex. Here, we show that human MPT synthase and ATAC inhibited PKR, a double-stranded RNA-dependent protein kinase, to facilitate translation initiation of ...

In this paper we complete the work begun by Mendelsohn and Rosa and by Hartman, finding necessary and sufficient conditions for a maximum packing with triples of order m MPT(m) to be embedded in an MPT(n). We also characterize when it is possible to embed an MPT(m) with leave LI in an MPT(n) with leave L2 in such a way that L1 C L2.

Mycobacterium paratuberculosis (MPT) is the etiologic agent of paratuberculosis. The disease is prevalent in cattle worldwide, and exacts a heavy financial toll. Effective control requires the development of acellular vaccines offering a better protection than the current available vaccines without side effects and allowing the discrimination between infected and vaccinated animals. We studied ...

The role of the mitochondrial permeability transition (MPT) in the killing of HeLa cells by staurosporine (STR) was assessed with the use of bongkrekic acid (BK), an inhibitor of the MPT. BK prevented cell killing as well as biochemical manifestations of the MPT: (a) the loss of the mitochondrial membrane potential (DCm); (b) the release of cytochrome c from the intramembranous space to the cyt...