A high-performance protocol for callus induction was devised using germinating mature embryos of two local wheat (Triticum aestivum L.) landraces as explant. The results showed that development from rapid starting one day after culture with an rate 20 to 25% higher than those soaked embryos. In addition, the mean growth developed 60 70% cultured This study also demonstrated a frequency green sp...

Lotus (Nelumbo nucifera Gaertn.) seed proteome has been the focus of our studies, and we have recently established the first proteome dataset for its mature seed endosperm. The current study unravels the immature endosperm, as well as the embryo proteome, to provide a comprehensive dataset of the lotus seed proteins and a comparison between the mature and immature endosperm tissues across the s...

The purpose of the research study is to select the best rice explant and variety for Agrobacterium mediated genetic transformation studies. In vitro callus induction and regeneration frequency of two explants i.e. immature embryo and mature grains of 9 rice varieties (Oryza sativa L.) was investigated by culturing explants source on N6 media supplemented with 2, 4-D at 2mg/l for callus inductio...

plant regeneration was achieved in verbascum speciosum schard. via organogenesis and somatic embryogenesis by culture of mature embryo explants. two types of calli, embryogenic and non-embryogenic, were induced from mature embryo explants on murashige and skoog (ms) medium supplemented with different concentrations of benzyl adenine (ba) and α-naphthalene acetic acid (naa). in order to further ...

OBJECTIVE To investigate fertilization and embryo quality of dysmorphic mature oocytes with specific morphological abnormalities obtained from intracytoplasmic sperm injection (ICSI). METHODS The fertilization rate (FR) and embryo quality were compared among 58 dysmorphic and 42 normal form oocytes (control 1) obtained from 35 consecutive ICSI cycles, each of which yielded at least one dysmor...

OBJECTIVE To report the arrest of euploid embryos with high mtDNA content. DESIGN A report of 2 cases. SETTING Private fertility clinic. PATIENTS 2 patients, 45 and 40 years old undergoing IVF treatment. INTERVENTIONS Mature oocytes were collected and vitrified from two ovarian stimulations. Postthaw, survived mature oocytes underwent fertilization by intracytoplasmic sperm injection (I...

A positive selectable marker system was adapted for transformation of mature embryo-derived calli of Indica rice (Oryza sativa L.) utilizing the PMI gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. The transformed cells grew on medium supplemented with 3% mannose as carbon source and calli were selected on media containing various concentrati...

For sexual reproduction of Allium hirtifolium, collected bulbs were planted in the field. In order to develop ovary embryo and seed setting, control self-pollination and natural open pollination took placed using honey bees under cages. Ovary development and seed setting of A. hirtifolium extraordinary were affected by pollination and there was a significant difference between mean effect of se...