Guar galactomannan, a naturally occurring polysaccharide, is susceptible to hydrolysis by three enzymes: beta-mannosidase, beta-mannanase, and alpha-galactosidase. The beta-mannosidase cleaves a single mannose unit from the nonreducing end of the guar molecule, the beta-mannanase cleaves interior glycosidic bonds between adjacent mannose units, and the alpha-galactosidase cleaves the galactose ...

A total of 288 crossbred (Duroc×Landrace×Yorkshire) growing pigs were used in two experiments to investigate the effects of adding β-mannanase to corn-soybean meal-based diets on pig performance and apparent total tract digestibility (ATTD). Both experiments lasted 28 d and were split into two phases namely 1 to 14 days (phase 1) and 15 to 28 days (phase 2). In Exp. 1,144 pigs weighing 23.60±1....

Aspergillus terreus FBCC 1369 was grown in solid-state culture under statistically optimized conditions. β-Mannanase was purified to apparent homogeneity by ultrafiltration, anion exchange and gel filtration chromatography. A purification factor of 10.3-fold was achieved, with the purified enzyme exhibiting specific activity of 53 U/mg protein. The purified β-mannanase was optimally active at p...

This study was conducted to evaluate the effect of dietary β-mannanase supplementation and palm kernel meal (PKM) inclusion (5%) on laying performance, egg quality and nutrient utilizability of laying hens with 73 weeks of age. A total of 240 Lohmann brown laying hens with average 77.5% egg production were randomly allocated with 60 hens per treatment, 4 replicates per treatment and 15 hens per...

A bacterium, MAN-511 that produced extracellular mannanase, was isolated and identified as Bacillus subtilis on the basis of 16S rDNA phylogenetic analysis. The enzyme was purified to apparent homogeneity by precipitasi ammonium sulfat 70% and Sephadex G-75 chromatography procedures. The mannanase was purified 7.6 fold and specificity of 9.3 U/mg protein. SDS-PAGE of the purified enzyme showed ...

Thermophilic mannanase from the Bacillus sp. MG-33 was applied on wheat straw-rich-soda pulp to evaluate its bleaching potential. Optimum conditions for bio-bleaching of pulp were found as, mannanase 3Ug -1 of pulp at pH 6.5 and temperature 65oC that enhanced the brightness by 30% and reduced the kappa number by 20% within 2h. Tear index (1.1, points) and burst index (0.02, points) were also im...

OBJECTIVE This experiment was conducted to determine the effects of dietary β-mannanase on the additivity of true metabolizable energy (TME) and nitrogen-corrected true metabolizable energy (TMEn) for broiler diets. METHODS A total of 144 21-day-old broilers were randomly allotted to 12 dietary treatments with 6 replicates. Five treatments consisted of 5 ingredients of corn, wheat, soybean me...

BACKGROUND Mannanase is an enzyme that can catalyze random hydrolysis of beta-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans which are the key polymers in hemicellulose. It has been used in a number of different industrial applications including food, feed, pharmaceutical, pulp/paper industries, and second generation biofuel. To optimize the expression sys...

Beta-mannanase otherwise known as mannan endo-1,4-βmannosidase or 1,4-β-D-mannanase with an enzyme commission (EC) 3.2.1.78 breakdowns β-1,4-mannosidic bonds in the main chain of glucomannans, galactomannans and β-1,4-mannans [1]. It transforms the abundant mannan-rich heteromannan, glucomannan and galactomannan substrates into mannooligosaccharides [2] and a small amount of mannose, glucose an...

BACKGROUND Mannans and heteromannans are widespread in plants cell walls and are well-known as anti-nutritional factors in animal feed. To remove these factors, it is common practice to incorporate endo-β-mannanase into feed for efficient nutrition absorption. The objective of this study was to overexpress a β-mannanase gene directly in maize, the main ingredient of animal feed, to simplify the...