The coagulation cofactor Va (FVa) is a noncovalent heterodimer consisting of a heavy chain (FVaH) and a light chain (FVaL). Previously, the fibrinolytic effector plasmin (Pn) has been shown to inhibit FVa function. To understand this mechanism, the fragmentation profile of human FVa by Pn and the noncovalent association of the derived fragments were determined in the presence of Ca(2+) using an...

Abstract Background The two-spotted spider mite, Tetranychus urticae Koch (Trombidiformes: Tetranychidae), is one of the most damaging mites in agriculture. Due to concern for intensive use synthetic acaricides, entomopathogenic fungi represents a feasible alternative T. management. In present study, 7 isolates Metarhizium were characterized physiological and molecularly (based on ITS1-5.8s-ITS...

The diverse forms of protein phosphatase 1 in vivo result from the association of its catalytic subunit (PP1c) with different regulatory subunits, one of which is the G-subunit (G(M)) that targets PP1c to glycogen particles in muscle. Here we report the structure, at 3.0 A resolution, of PP1c in complex with a 13 residue peptide (G(M[63-75])) of G(M). The residues in G(M[63-75]) that interact w...

Blue-pigmented exudates arise as droplets on sporulated lawns of Streptomyces coelicolor M110 grown on agar plates. Our electron microscopical and biochemical studies suggest that droplets contain densely packed vesicles with large assemblies of different protein types and/or the polyketide antibiotic actinorhodin. Frozen-hydrated vesicles were unilamellar with a typical bilayer membrane, and r...

Given positive integers m, k and s with m > ks, let Dm,k,s represent the set {1, 2, · · · ,m} − {k, 2k, · · · , sk}. The distance graph G(Z,Dm,k,s) has as vertex set all integers Z and edges connecting i and j whenever |i − j| ∈ Dm,k,s. The chromatic number and the fractional chromatic number of G(Z,Dm,k,s) are denoted by χ(Z,Dm,k,s) and χf (Z,Dm,k,s), respectively. For s = 1, χ(Z,Dm,k,1) was s...

The genetic ancestry of Polynesians can be traced to both Asia and Melanesia, which presumably reflects admixture occurring between incoming Austronesians and resident non-Austronesians in Melanesia before the subsequent occupation of the greater Pacific; however, the genetic impact of the Austronesian expansion to Melanesia remains largely unknown. We therefore studied the diversity of nonreco...

Functionalized single-wall carbon nanotubes (f-SWCN) are dispersed in chitosan films by self-assembly of both components aqueous media. This form is a promising nanofiller to achieve nanocomposites with refined thermal, mechanical and surface features. In this study, we investigated the influence functionalized concentration on resulting properties structures. molecular scale polymeric matrix d...