Background: Despite several studies many questions remain regarding how varicocele develops its adverse effect on testicular tissue. Spermatogenesis cell lineage needs carbohydrates as a main source of energy for cell division. Any disruption in glucose transporting system in seminiferous tubules results in remarkable decrease in germinal cells biological function. Therefore present study was c...

enzyme efficiency was investigated in the lipase-catalyzed hydrolysis of canola oil in supercritical carbon dioxide (scco2). immobilized lipase from mucor miehie (lipozyme im) was used as the catalyst and the results showed that enzyme efficiency dropped at high pressures indicating a possible change in enzyme microstructure. therefore, scanning electron microscopy (sem) was used as a supplemen...

conclusions results of this study could be used to find suitable targets for inverse metabolic engineering and site-directed mutagenesis to achieve stable and high-level extracellular lipase production in y. lipolytica and other yeasts. results two nucleotide substitutions were found in the extracellular lipase lip2 orfs of y. lipolytica u6 mutant strain compared to the wild-type strain. in thi...

background and objectives: lipases are valuable biocatalysts which are widely used in the detergent, food, dairy and pharmaceutical industries. the aims of the present study included the isolation of a lipase-producer from industrial zones and the partial characterization of the enzyme. materials and methods: a number of bacteria were isolated from sites related to the oil industries. an isolat...

pseudomonas aeruginosa ef2, atcc 9027 and atcc 19660 were grown in a continuous culture under tween 80 (polyoxyethylene sorbitan monooleate) limitation and optimum conditions (ph 6.5, 37°c at dilution rate of 0.05/h). culture supernatants were carefully removed and stored at -20°c. to purify the lipases, the culture supernatant was reduced in volume to approximately 10 ml by an ultrafiltration ...

Background: Lipase is an enzyme with immense application potential. Ester synthesis by lipase catalysis in organic media is an area of key industrial relevance. Enzymatic preparations with traits that cater to the needs of this function are hence being intensely researched. Objective: The objectives of the study were to immobilize the lipase from Bacillus sp. PS35 by cross-linking and adsorpti...

Lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. In this study, an alkaline thermostable lipase producing bacteria were isolated from soil of different regions of Isfahan province (Iran) and its lipase was purified by ammonium sulfate precipitation and ion exchange chromatography. To select a the...

To avoid the secondary pollution that inorganic corrosion inhibitor may cause in circulating cooling water, the bio-corrosion inhibition effect of lysozyme, catalase, lipase, and laccase, as the biologic inhibitors without the above problem, and the increased bio-corrosion inhibition effect of Ca+2 and Mg+2 are studied. An enzyme with corrosion properties was selected by rotary coupon test. The...

Pseudomonas aeruginosa EF2, ATCC 9027 and ATCC 19660 were grown in a continuous culture under Tween 80 (polyoxyethylene sorbitan monooleate) limitation and optimum conditions (pH 6.5, 37°C at dilution rate of 0.05/h). Culture supernatants were carefully removed and stored at -20°C. To purify the lipases, the culture supernatant was reduced in volume to approximately 10 mL by an ultrafiltra...