BACKGROUND Lectins are carbohydrate binding proteins or glycoproteins that bind reversibly to specific carbohydrates present on the apposing cells, which are responsible for their ability to agglutinate red blood cells, lymphocytes, fibroblasts, etc. Interest in lectins has been intensified due to their carbohydrate specificity as they can be valuable reagents for the investigation of cell surf...

The mechanism underlying molecular recognition of lectins was elucidated by a novel solid phase binding assay system based on surface plasmon resonance. When the apparent affinities of interactions between chitooligosaccharides and wheat germ agglutinin were compared between lectin-immobilized and oligosaccharide-immobilized assay systems, the affinity constants (Ka) calculated for the former s...

Liver disease, in the form of hepatocellular carcinoma (HCC) accounts for > 700,000 deaths worldwide. A major reason for this is late diagnosis of HCC. The currently used biomarker, serum alpha-fetoprotein (AFP) is elevated in 40-60% of those with HCC and other markers that can either compliment or replace AFP are desired. Our previous work has identified a number of proteins that contain alter...

b a ckground: the role of lectins is important in interaction between pathogens and mosquito vectors. this study was performed to identify agglutinin activities of protein molecules on the midgut of culex pipiens . m e t hods: c u lex pipiens was reared in insectray condition and the midguts of males and females (blood fed and un- fed) were dissected separately in tris-hcl buffer. the extracts ...

BACKGROUND Galactose-deficient IgA1 (Gd-IgA1) is a critical effector molecule in the pathogenesis of IgA nephropathy (IgAN). Although many researchers have measured serum levels of Gd-IgA1 using snail helix aspersa agglutinin (HAA) lectin-based assay, the lectin-dependent assay has some serious problems in robustness. In this study, we aimed to establish a more robust and stable enzyme-linked i...

The Present study demonstrates the diagnostic characteristics of Pedilanthus tithymoloides, agglutinin (PTA), lectin based hemagglutination method for screening of thyroid disorders as compared with RIA as gold standard. The result estimates 90% sensitivity, 95.55% specificity and 92.63% observed agreement with RIA test in thyroid disorders. The result of hyperthyroidism, hypothyroidism and cre...

Membrane fractions from seedlings of four soybean [Glycine max (L.) Merr.] lines were examined by radioimmunoassay and hemagglutination assay for the 120,000 dalton soybean lectin. Two of the lines (Sooty and T-102) are genotypically lele and lack buffer-soluble soybean lectin; the remaining two lines (Beeson and Harosoy 63) are Le and produce seeds that contain the lectin (Su et al. 1980 Bioch...

We constructed an assay system for the rapid detection of the glycosylation status of antibodies with a chemiluminescence-based lectin-binding assay, and investigated the glycosylation dynamics during the culture of recombinant Chinese hamster ovary cells. This rapid detection system is applicable to the high-throughput evaluation of therapeutic glycoproteins.

An enzyme-linked lectin assay (ELLA) has been developed to detect specific carbohydrate units on the surface of unfixed cells. The assay may be read in standard ELISA plate readers, since the cell-bound enzyme-lectin conjugate is specifically eluted from the cells prior to development of the conjugate. ELLA, when read in an enzyme-linked immunosorbent assay (ELISA) plate reader, allows better d...

Early detection of cancer disease is vital to the successful treatment, follow-up and survival patients, therefore sensitive specific methods are still required. Mucin 1 (MUC1) a clinically approved biomarker for determining that type I transmembrane protein with dense glycosylated extracellular domain extending from cell surface 200–500 nm. In this study, nanopolymers were designed lectin affi...