The use of ?-lactamase inhibitors in combination with ?-lactam antibiotics is an emerging area drug discovery. This strategy allows the restoration therapeutic efficacy these clinical against multiresistant bacteria. These pathogens are resistant because they express enzymes, which prevent antibiotic action by catalyzing hydrolysis ring. enzymes quite diverse both their structural architecture ...

Although β-lactams have been the most effective class of antibacterial agents used in clinical practice for the past half century, their effectiveness on Gram-negative bacteria has been eroded due to the emergence and spread of β-lactamase enzymes that are not affected by currently marketed β-lactam/β-lactamase inhibitor combinations. Avibactam is a novel, covalent, non-β-lactam β-lactamase inh...

Using a nitrocefin competition assay, I determined the relative substrate affinity index (RSAI) values of nine clinically significant beta-lactamase enzymes against a range of beta-lactams. Using selected beta-lactam substrates, I observed large differences in the RSAI values of the nine enzymes that were sufficient in many cases to positively identify specific enzymes. I made use of the unique...

β-lactamases are enzymes which inactivate the β-lactam antibacterial agents and are one of the major causes of resistance against these drugs. Recently there are reports on the isolation of bacteria which does not produce β-lactamase, but are resistant to penicillins. In the present study, β-laclamase production was determined using iodometric method on 150 ampicillin resistance Escherichia col...

conclusions regarding the importance of metallo-beta-lactamase (mbl) produced by pseudomonas spp. isolated from hospitals, quick identification of clinical samples should be considered as an important and basic step to treat and control infections caused by pseudomonas spp. results strains with mic > 4 µg/ml were investigated by phenotypic and genotypic methods. in the current study the rate of...

The goal of this study was to isolate and identify Staphylococcus aureus from clinical environmental sources. This also looked at the prevalence distribution different virulence enzymes among its isolates. For objective, 65 samples were collected dispersed 50 distinct 15 (soil, water, air) samples. results revealed that 20 bacterial isolates S. aureus. isolation rate 30.76% all samples, with 13...

Background and Objective: Biofilm formation plays a crucial role in wound infections and increases the bacteria resistance to treatment. The present study investigated the relationship between the biofilm formation, ESBL, AmpC, and KPC enzymes in Acinetobacter baumannii isolated from the wound specimens. Materials and Methods: Eighty- nine A. baumannii isolates were collected from wound specim...

Four novel oxapenem compounds (i.e., AM-112, AM-113, AM-114, and AM-115) were investigated for their beta-lactamase inhibitory activity against a panel of isolated class A, C, and D enzymes, which included expanded-spectrum beta-lactamase enzymes (ESBLs). The oxapenems were potent beta-lactamase inhibitors. Activity varied within the group, with AM-113 and AM-114 proving to be the most active c...

The interactions between imipenem and four monobactams and three class A beta-lactamases have been studied in detail. Despite their reputation as being beta-lactamase-stable, some of these compounds were significantly hydrolysed by the enzymes. The results obtained with the Streptomyces albus G beta-lactamase have been analysed in the light of molecular modelling studies. The discussion is exte...

Proteus mirabilis is a specific opportunistic pathogen of many infections including urinary tract (UTIs). Risk factors are linked with the acquisition multidrug-resistant (MDR) to 3 or more classes antimicrobials) strains. The resistance in extended-spectrum alpha-lactamase rare, but rising beta-lactamase (ESBL) producing strains matter concern. ?-lactamases and antibiotic modifying enzymes mai...