Genetic and epigenetic changes (DNA methylation) in the mother plants of somatic hybrids (1st cycle) and their regenerants (30th cycles sub-cultured) were analyzed using methylation-sensitive ISSR and RAPD markers. ISSR and RAPD profiles and their cluster analyses based on the Jaccard’s similarity coefficient revealed 100% genetic similarity among the mother plant and their regenerants. While f...

Gypsophila L. is the 3rd-largest genus of Caryophyllaceae in Turkey, a country that includes 60 taxa belonging to 56 species of the 126 recognized in the genus. A total of 35 taxa are endemic to Turkey, with an approximately 60% endemism ratio. In this study, the genetic diversity of 14 Gypsophila species from Turkey was analyzed using random amplification of polymorphic DNA (RAPD) and inter-si...

DNA based molecular markers allow precise, objective and rapid cultivar identification. In this study, five ISSR and two sets of fluorescence-labeled TP-M13-SSR markers were applied for discrimination of eight closely related wheat cultivars. Five ISSR primers revealed a total of 43 distinct reproducible bands, 29 (67.44%) of the 43 bands were polymorphic. The number of polymorphic bands detect...

Genetic variations of 15 Brahmi (Bacopa monnieri L.) accessions were evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. During RAPD analysis, amplification of genomic DNA of the 15 accessions by 22 primers generated 197 fragments, of which 187 were polymorphic with an average of 8.95 bands per primer. The amplified products varied in size f...

Inter-simple sequence repeat (ISSR) analysis was used to develop fish species-specific molecular markers for four Tilapia species (Oreochromis niloticus, Oreochromis aureus, Tilapia zillii and Sarotherodon galilaeus). 12 ISSR primers were tested to assess the effectiveness of ISSR analysis in discriminating among the four applied fish species. Some ISSR markers were detected as species-specific...

 DNA markers are integrally connected to the success of molecular breeding and are fundamentally required by breeders to be able to, a. identify new gene sources in the available biodiversity, b. select parents in order to increase heterosis, c. decrease the number of backcross generations for gene introgression breeding programs, and d. carry out marker-assisted selection (MAS). The present re...

Date palm (Phoenix dactylifera L.) is a monocot, dioecious and high longevity tropical plant greatly considered due to its high economic importance in Middle East. This investigation was carried out to detect genetic relations among 34 cultivars of Iranian date palm, both males and females, using ISSR markers. Accordingly, total DNA was extracted using CTAB method and was subjected to PCR ampli...

Twelve species belonging to six fungal genera were found to be associated with wheat (Triticum aestivum L.) grain samples collected from three main regions in Saudi Arabia. The most common genera (average frequency) were Aspergillus (14.3%), Fusarium (29.1%), Penicillium (9.3%), and Alternaria (8.2%). Nineteen isolates of Aspergillus flavus were screened for their ability to produce aflatoxins ...

the effectiveness of irap, remap, ssr, and issr markers were investigated to assess genetic diversity among and within eight medicago sativa l. populations. a total of 101, 119, 117 loci and 31 alleles were amplified using 10 irap, 14 remap, 16 issr and eight ssr primers, respectively. irap markers generated the maximum proportion of polymorphic loci per primer (pplp) while the maximum value of...

Eucommia ulmoides Oliver, one of the tertiary relict species found only in China, is the only extant species of Eucommiaceae. Using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers, we studied the genetic diversity and population genetic structure of 187 accessions from 17 E. ulmoides populations throughout its main distribution in China. A total of...