The present study was undertaken to investigate the interactions among the supplies of isoleucine, leucine, valine, and tryptophan in laying hens. A three-factor trial was conducted with laying hens in which the dietary concentrations of isoleucine (5.7, 8.0, and 11.5 g/kg), valine and leucine (6.3 and 7.2 g/kg and 10.1 and 11.5 g/kg, respectively), and tryptophan (1.5 and 2.4 g/kg) were varied...

In separate experiments the isoleucine and leucine requirements of the kitten were determined on the basis of growth and nitrogen retention. The dietary concentrations of isoleucine tested were (g/kg diet) 1.4, 2.2, 3.0, 3.8, 4.6 and 9.0 with adequate (12.0 g/kg diet) leucine. The levels of leucine tested were (g/kg diet) 5.0, 7.5, 9.0, 10.5, 12.0 and 20.0 in diets containing adequate (9.0 g/kg...

Peptide histidine isoleucine, a 27 amino acid peptide with close amino acid sequence homology to vasoactive intestinal peptide and secretin, is distributed throughout the mammalian intestinal tract, where it has been localised to intramural neurones. An intestinal perfusion technique has been used to study the effect of intravenous peptide histidine isoleucine (44.5 pmol/kg/min) on water and el...

This study conducted to evaluate the effect of prebiotics (Fermacto) and isoleucine and their combination on performance and some blood parameters of broiler chickens during finisher period. At the first of experiment 300 one-day-old mixed Ross broiler chicks reared in same condition according to Ross manual guide up to 21 days old. Chicks at 21 day old were randomly allocated to 20 groups of 1...

Knowledge of regulation of glucose transport contributes to our understanding of whole-body glucose homoeostasis and human metabolic diseases. Isoleucine has been reported to participate in regulation of glucose levels in many studies; therefore, this study was designed to examine the effect of isoleucine on intestinal and muscular GLUT expressions. In an animal experiment, muscular GLUT and in...

The catabolic or biodegradative threonine dehydratase (E.C. 4.2.1. 16) of Escherichia coli is an isoleucine feedback-resistant enzyme that catalyzes the degradation of threonine to alpha-ketobutyrate, the first reaction of the isoleucine pathway. We cloned and expressed this enzyme in Corynebacterium glutamicum. We found that while the native threonine dehydratase of C. glutamicum was totally i...

L-Isoleucine was discovered in 1904 by Ehrlich (l), and its constitution established 3 years later (a) by degradation to d-isoamylamine and (b) by synthesis through the Strecker reaction with d-isovaleraldehyde (2). The synthetic product, however, appeared on fractional crystallization to be optically inhomogeneous, and Ehrlich attributed this to the presence in nearly equal parts of natural is...

Isoleucine, a branched chain amino acid, plays an important role in the improvement of glucose metabolism as evidenced by the increase of insulin-independent glucose uptake in vitro. This study evaluated the effect of isoleucine on glucose uptake and oxidation in fasted rats and on gluconeogenesis in vivo and in vitro. Oral administration of isoleucine decreased the plasma glucose level by 20% ...

Cyclopentaneglycine (CPG) inhibited the growth of wild-type Salmonella typhimurium. The inhibition was overcome by isoleucine or any isoleucine precursor formed after threonine. CPG appeared to mimic isoleucine as a strong inhibitor of the activity of l-threonine deaminase. The analogue was a poor inhibitor of isoleucyl-transfer ribonucleic acid synthetase. CPG did not appear to be incorporated...