the use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. fifty to one hundred µm silver thiosulfate (sts) produced a larger leaf area as a source of plant material for protoplast isolatio...

The aim of this paper is to present several popular methods for in vitro culture of osteocytes and osteocyte cell lines. Osteocytes are located extremely suitably within the calcified bone matrix to sense mechanical signals, and are equipped with a multitude of molecular features that allow mechanosensing. However, osteocytes are more than specialized mechanosensing cells. Several signaling mol...

The liver plays a major role in in vivo metabolisms such as in synthesis of plasma proteins, blood glucose generation, urea formation, secretion of bile, and detoxification. Since most of these hepatic functions are performed by hepatocytes, parenchymal cells of the liver, there has been much attention to their use for cell therapeutic applications and the in vitro studies of liver physiology, ...

The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, which is located between the basal lamina and sarcolemma of muscle fibers and is the primary source of myogenic precursor cells in postnatal muscle. Muscle satellite cells are a useful model to invest...

conclusions this paper may serve as a guide to screening, isolation, and cultivation of more new mtb. the new isolated strain opens up good opportunities for biotechnological applications such as medicine to bioremediation processes due to its unique abilities. results the results revealed a correlation of important physicochemical factors such as ph and iron with growth and blooms of these bac...

background brucellosis is a zoonotic disease that is endemic in iran. appropriate and rapid diagnosis has a vital role in public health improvement. low isolation rate of the organism has reported frequently in various reports. the present study was conducted to determine the isolation rate of organism in culture from collected specimens of hospitalized patients who were not under antibiotic th...

objective: among the members of legionellaceae, legionella pneumophila is involved in 95% of cases of severe pneumonia. isolation of the causative agent from bronchoalveolar lavage (bal) fluid specimen is a delicate process and also time-consuming. moreover, it has been shown that some legionella strains may be viable but cannot be cultured. the aim of this study was comparison of culture and p...

Organ cultures of human embryonic trachea in test tubes were used as an adjunct to tissue cultures in the isolation of respiratory viruses from children in hospital. Fifty-one viruses were obtained from 127 specimens, giving an isolation rate of 40%. Fifteen viruses were isolated from the original tissue cultures and also after passage through organ culture. Thirty viruses were isolated from th...