This article provides an analysis on published models used to calculate phase separation in aqueous two-phase systems (ATPS) based Flory-Huggins (FH) theory, terms of problem formulation and mathematical solving algorithm. An integrated algorithm is presented, showing different approaches using the FH theory. The involves estimation interchange energy, calculation compositions. Based experiment...

Heavy water has been used as a tool to study the role of water in the association-dissociation equilibrium of subunit proteins. In the case of lactate dehydrogenase, DzO was found to stabilize the enzyme at low ionic strength and inhibit subunit interchange between heartand muscle-type enzyme in solution. When frozen, however, subunit interchange occurred to a similar extent in either DzO or Hz...

Heavy water has been used as a tool to study the role of water in the association-dissociation equilibrium of subunit proteins. In the case of lactate dehydrogenase, DzO was found to stabilize the enzyme at low ionic strength and inhibit subunit interchange between heartand muscle-type enzyme in solution. When frozen, however, subunit interchange occurred to a similar extent in either DzO or Hz...

Intrinsically Disordered Proteins (IDPs) are enriched in charged and polar residues; and, therefore, electrostatic interactions play a predominant role in their dynamics. In order to remain multi-functional and exhibit their characteristic binding promiscuity, they need to retain considerable dynamic flexibility. At the same time, they also need to accommodate a large number of oppositely charg...

The traditional method of pairwise job interchange compares the cost of sequences that differ only in the interchange of two jobs. It assumes that either there are no intermediate jobs (adjacent pairwise interchange) or that the interchange can be performed no matter what the intermediate jobs are ( nonadjacent pairwise interchange). We introduce a generalization that permits the pairwise inter...