BACKGROUND The aim of this study was the ex vivo expansion of Umbilical Cord Blood hematopoietic stem cells on biocompatible nanofiber scaffolds. MATERIALS AND METHODS CD133+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system by means of monocolonal antibody CD133 (microbeads); subsequently, flowcytometry method was done to assess the ...

The Effects of Neuropeptide Y on the invitro mulhunit activity rhythm in the suprachiasmatic nucleus" (1997). University of Tennessee Honors Thesis Projects.

Six digestion trials were conducted using eight Suffolk rams (four/trial; two trials run concurrently) to determine the ability of 11 techniques to estimate in vivo apparent DM digestibility (DMD). Diets (trials) were as follows: 1) ad libitum access to chopped fescue hay, 2) ad libitum access to alfalfa hay, 3) limit-fed fescue hay, 4) limit-fed alfalfa hay, 5) 25% soybean meal and 75% fescue ...

immediate early step in PrE specification. We show that the fraction cells present with in this state is influenced by factors that both promote and suppress primitive PrE differentiation, however conditions that support ES cell self-renewal prevent their progression into endoderm differentiation and support an equilibrium between this state and at least one other that resembles the inner cell ...

The antioxidant effect of N, N-dimethylglycine (DMG) on in vitro-produced (IVP) bovine embryos was examined. After in vitro fertilization, presumptive zygotes were cultured with or without 0.1 μM DMG under different oxygen tensions. The percentage of embryos developing to the blastocyst stage was lowest under a 20% oxygen concentration without DMG, and it was significantly increased (P < 0.05) ...

Introduction: Osteoarthritis (OA) is a chronic, debilitating, degenerative joint disease in which degradation of the ECM macromolecules aggrecan and type II collagen are a major feature. Invitro models of degradation using Oncostatin M (OSM) in combination with Interleukin-1β (IL-1) stimulate collagen breakdown in some species [1, 2]. However, in our preliminary findings, this was not observed ...

immediate early step in PrE specification. We show that the fraction cells present with in this state is influenced by factors that both promote and suppress primitive PrE differentiation, however conditions that support ES cell self-renewal prevent their progression into endoderm differentiation and support an equilibrium between this state and at least one other that resembles the inner cell ...

immediate early step in PrE specification. We show that the fraction cells present with in this state is influenced by factors that both promote and suppress primitive PrE differentiation, however conditions that support ES cell self-renewal prevent their progression into endoderm differentiation and support an equilibrium between this state and at least one other that resembles the inner cell ...

During healing of a skin wound, cells that are normally dormant must turn on hundreds of genes in order to proliferate, migrate, and repair the defect. The Polycomb family of proteins epigenetically silence target genes and so downregulation of their expression is a potential mechanism for unsilencing repair genes. Quantitative RT-PCR, western blotting, and immunostaining of invitro epithelial ...

  Proteases play a key role in protein digestion in ticks and other haematophagous insects. Our understanding of blood meal digestion in digestive system of ticks can be very useful for better understanding of basic rules for control of ticks. Cells of the midgut endocytose blood components. Blood proteins uptake by midgut cells, suggesting the presence of proteases in the midgut cells. In this...