the study was undertaken to elucidatethe beneficial effects of goat follicular fluid (gff) added to maturation and culture media on in vitro maturation, fertilization and post-fertilization development of oocytes in black bengal goats. follicular fluid and oocytes were collected from slaughter house goat’s ovaries using the aspiration technique. media were prepared using tcm-199 supplemented wi...

purpose: to evaluate the effect of human amniotic fluid (haf) on the growth of human corneal endothelial cells (hcecs) and to establish an in vitro method for the expansion of hcecs. methods: hcecs were cultured in dmem-f12 supplemented with 20% fetal bovine serum (fbs). confluent monolayer cultures were trypsinized and passaged using 20% fbs- or 10%, 20%, or 30% haf-containing media. cell prol...

Stem cells, on regenerative medicine, has enormous potential and impact, lead to the rapidly growing interest for tools to analyze and characterize the behaviors of these cells invitro in an automated and high throughput fashion. Measurement of the proliferative behaviors of cells invitro is important to many biomedical applications for the measurement of the accurate counting and localization ...

Primordial germ cells (PGCs) are the precursors of the germ cell lineage. A method for the prolonged invitro culture of chicken PGCs, which retain the ability to colonise the gonad when introduced into developing embryos, has been described (van de Lavoir etal., Nature 441 (2006) 766–769). The invitro culture of PGCs provides a useful tool for the creation of transgenic chickens and investigati...

Primordial germ cells (PGCs) are the precursors of the germ cell lineage. A method for the prolonged invitro culture of chicken PGCs, which retain the ability to colonise the gonad when introduced into developing embryos, has been described (van de Lavoir etal., Nature 441 (2006) 766–769). The invitro culture of PGCs provides a useful tool for the creation of transgenic chickens and investigati...

Primordial germ cells (PGCs) are the precursors of the germ cell lineage. A method for the prolonged invitro culture of chicken PGCs, which retain the ability to colonise the gonad when introduced into developing embryos, has been described (van de Lavoir etal., Nature 441 (2006) 766–769). The invitro culture of PGCs provides a useful tool for the creation of transgenic chickens and investigati...

Rumex vesicarius L .commonly known as” Bladder dock or Chukkakura” is a renowned medicinal plant, which belongs to the family polygonaceae.It posses anti inflammatory, antimicrobial, analgesic, antiviral, antioxidant, cordial and stomachache properties. An attempt has been made to study clonal propagation of this important medicinal plant from nodal segments as explants. These explants were ino...

modern biotechnology and genetic engineering using techniques and special ‎laboratory tools plays an important role in the pharmaceutical industry and medicinal ‎plant propagation. among these techniques, it can be noted that a wide range of ‎elicitors are widely used for inducing metabolites at invitro culture. due to the ‎pharmaceutical properties of artichoke and its history, it has been dee...

The present study was designed with an objective to assess the inhibition of lipid peroxidation (LPO) by the aqueous extract of Calocybe indica (milky mushroom) using an invitro model of goat liver homogenate and RBC ghosts. The invitro LPO was inhibited to a good extent by the aqueous extract of milky mushroom and the extent of inhibition being higher in the RBC membrane model when compared wi...

The antagonistic potential of culture filtrates of a soil isolate of T. viride was evaluated invitro, against three common post-harvest rot pathogens; A. niger, A. flavus, and F. oxysporum. Assay of three extracellular enzymes; chitinase, protease and cellulase, in respective enzyme-induced culture filtrates revealed the presence of enzyme activity. When 10% (v/v) concentration of the various l...