Pyran copolymer (NSC 46015) was found to potentiate strongly the immune response of C57BL/6J X DBA/2 F1 mice to 10(4) live L1210 tumor cells following suboptimal vaccination with 10(7) radiation-inactivated L1210 cells. Optimal immunity to challenge was produced by concomitant i.p administration of pyran and L1210 vaccine, and activity was dependent upon both pyran and vaccine dosages. In addit...

Prime-and-boost vaccination strategy with DNA and protein vaccines is broadly adopted to augment the immunogenicity of both vaccines, but the mechanism is ambiguous. Antigen-specific immunological memories in humoral and cellular immune responses were examined in mice after immunization with different regimens, by the evaluation of persistence of antibody production and CTL activity, as well as...

Ovarian cancer remains the leading cause of death due to gynecologic cancer in women in the United States. Gene and viral-based therapies represent novel therapeutic approaches for cancer. The manipulation of genetic content of tumor cells toward a therapeutic end has been divided into several general strategies, including molecular chemotherapy, mutation compensation, immunopotentiation, and v...

Apoptosis is considered to be a way of eliminating unwanted cells without causing major inflammation. Nevertheless, several lines of evidence show that apoptotic cell-derived antigens can be strong immunogens. The rabies virus glycoprotein G-ERA is an apoptotic molecule. We tested the ability of G-ERA to potentiate a B cell response against an exogenous antigen (influenza hemagglutinin, HA). We...

UMEZAWA et al. found that forphenicine, isolated from culture filtrates of Actinomycetes fulvoviridis var. acarbodicus is a specific inhibitor against chicken intestine alkaline phosphatase and it was shown to enhance delayed type hypersensitivity and antibody formation in mice1,2). Thereafter, ISHIZUKA et al. found that forphenicinol, a synthetic derivative of forphenicine, enhanced delayed ty...

A mouse strain (CB6) that is highly susceptible to Mycobacterium lepraemurium was infected with 10(8) bacilli into the hind footpad. These mice developed cell-mediated immunity to M. lepraemurium, as expressed by the development of a granulomatous lesion at the site of inoculation in normal but not in T-lymphocyte-depleted mice, a proliferative response in the paracortical zone of the draining ...

Cordyceps mycelium extract (Cs-4), Chicken Essence (CE), and their combination were investigated for antioxidant immunomodulatory activities in mouse models. Long-term treatment with Cs-4 or CE at equivalent human doses improved status various tissues, as evidenced by the enhancement of mitochondrial glutathione redox brain, liver, heart, kidney mice. also produced an action, indicated potentia...