Hypoallergenic formulas containing hydrolyzed protein are intended for use by infants to prevent cow’s milk allergy. The degree of hydrolysis epitopic areas determines the residual allergenicity whey hydrolysates (WPHs). However, only amino-acid-based infant (IFs) considered entirely nonallergenic. aim this study was investigate four commercially available WPHs with different (H1–H4) potential ...

BACKGROUND Genetically modified, (GM) crops with potential allergens must be evaluated for safety and endogenous IgE binding pattern compared to native variety, prior to market release. OBJECTIVE To compare endogenous IgE binding proteins of three GM maize seeds containing Cry 1Ab,1Ac,1C transgenic proteins with non GM maize. METHODS An integrated approach of in silico & in vitro methods wa...

Food allergens are mainly naturally-occurring proteins with immunoglobulin E (IgE)-binding epitopes. Understanding the structural and immunogenic characteristics of allergenic is essential in assessing whether how food processing techniques reduce allergenicity. We here discuss impacts technologies on modification physicochemical, structural, properties proteins. Detection for characterizing ch...

Background: Several studies reported the clinical features of IgE-mediated hypersensitivity after ingestion of melon. Melon allergy is a common IgE-mediated fruit allergy in Iran. This prompted us to investigate immunochemical and molecular properties of the major allergen in melon fruit, to compare the IgE-binding capacity of the natural protein with the recombinant allergen, and to determine ...

The IgE-binding proteins in beer were examined by immunoblotting analysis with sera of patients sensitive to beer. Several proteins were immunoblotted with the sera, and among these, 18-kDa proteins were identified as new IgE-binding proteins in beer. Perhaps they originated from barley as a raw material.

The linear IgE-binding epitopes of non-specific lipid transfer proteins (nsLTP) from plants were predicted using a combination of predictive tools including (1) the hydropathic profiles based on different scales of hydrophilicity, flexibility and exposure to the solvent, (2) the hydrophobic cluster analysis plots, (3) the occurrence of charged residues in the predicted amino acid sequence stret...

The cloning of recombinant proteins offers the possibility ofproducing, identifying, and manipulating the important IgE-binding determinants present on proteins that are responsible for provoking allergic diseases (1) . Proteins produced by mites of the genus Dermatophagoides are among the most important allergens affecting man in terms of both the frequency of the response by allergic subjects...

Background and Purpose: Allergy is an undesired immune response to non-pathogenic agents. However, some opportunistic microorganisms such as fungi can also cause allergy. Among those fungi, hyphae form of Aspergillus strains including A. fumigatus, A. flavus, and A. niger could be mentioned. In this study, we aimed to separate allergic proteins from Aspergillus strains and determine their ident...