نتایج جستجو برای: hirudin variant 3

تعداد نتایج: 1893080  

Journal: :Blood 1992
G Agnelli C Renga J I Weitz G G Nenci J Hirsh

Thrombus extension in patients with venous thromboembolism is due to the accretion of fibrin onto existing thrombi. Extension is promoted by both circulating and thrombus-bound thrombin, which convert fibrinogen to fibrin. Heparin is an effective antithrombotic agent, but it requires continuous administration to achieve persistent inhibition of thrombus extension. Heparin is highly effective in...

Journal: :Circulation 1998
K Riesbeck D Chen G Kemball-Cook J H McVey A J George E G Tuddenham A Dorling R I Lechler

BACKGROUND Intravascular thrombosis occurs in disorders of diverse pathogeneses, including allograft and xenograft rejection. In this in vitro study, we describe an approach for tethering the specific thrombin inhibitor hirudin to plasma membranes as part of a genetic strategy for regulating intravascular coagulation. METHODS AND RESULTS An HLA class I leader sequence was fused with hirudin l...

Journal: :medical laboratory journal 0
parisa bakhshi hamedan university of medical sciences, hamedan, iran massoud saidijam hamedan university of medical sciences, hamedan, iran delavar shahbazzadeh pasteur institute of iran, tehran, iran nazanin mohajerani pasteur institute of iran, tehran, iran hassan mirzahoseini pasteur institute of iran, tehran, iran

abstract         background and objective: hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. the aim of this study was to evaluate the effect of inserting protein a signal peptide sequence of pezz18 plasmid on expression and secretion of the recombinant hirudin in e.coli.  ...

Journal: :Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 1999
E Bucha R Kreml G Nowak

BACKGROUND After introducing the specific thrombin inhibitor recombinant hirudin (r-hirudin) into clinical practice in cases of heparin-induced thrombocytopenia (HIT, type II) the possibility of its use as an anticoagulant during haemodialysis treatment in HIT II patients is being discussed more frequently. On the one hand, the efficient, safe and routine use of r-hirudin during haemodialyses, ...

2006
Noriko Esumi Dominic Fan Isaiah J. Fidler

Recombinant desulfatohirudin (r-hirudin), a highly specific inhibitor of tImmillili, was examined to determine whether it would inhibit produc tion of experimental lung metastasis by B16-F10 melanoma cells. In in vitro assays using mouse plasma, the high level of procoagulant activity in B16-F10 cells was significantly inhibited by r-hirudin in a dosedependent manner. From 15 to 120 min after s...

Journal: :Clinical journal of the American Society of Nephrology : CJASN 2007
Kerstin Benz Matthias A Nauck Joachim Böhler Karl-Georg Fischer

Recombinant hirudin (lepirudin) is a potent direct thrombin inhibitor that is used particularly for treatment of immune-mediated heparin-induced thrombocytopenia. Because hirudin is almost exclusively eliminated by the kidneys, its half-life is markedly prolonged in patients with severe renal insufficiency. Therefore, these patients are at risk for bleeding, particularly because no antidote is ...

Journal: :Circulation 1994
P Zoldhelyi J Bichler W G Owen D E Grill V Fuster J S Mruk J H Chesebro

BACKGROUND The degree to which antithrombotic drugs suppress thrombin generation is unknown. Because hirudin, unlike antithrombin III, binds intravascular thrombin rapidly and selectively to yield a circulating inactive complex of 3- to 4-hour half-life, we used intravenous hirudin in humans to investigate the course of thrombin generation during and early after anticoagulation with this potent...

FT-IR and FT-Raman spectra of hirudin have been recorded from the native solid hirudin. The conformation of the molecule has been discussed on the basis of IR and Raman data. It has been concluded that hirudin molecule has a mixed a-helix and random coil conformation.

ژورنال: Medical Laboratory Journal 2015
Bakhshi , Parisa , Mirzahoseini, Hassan , Mohajerani, Nazanin , Saidijam, Massoud , Shahbazzadeh, Delavar ,

Abstract         Background and Objective: Hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. Recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. The aim of this study was to evaluate the effect of inserting protein A signal peptide sequence of pEZZ18 plasmid on expression and secretion...

Journal: :Clinical chemistry 1994
B Mille O Condamines J M Herbert M Maftouh C Picard D Dussossoy B Pau

Two monoclonal antibodies (mAbs), 10-2 and 10-5, both directed against recombinant hirudin variant 2-Lys47 (rHV2), were selected for their high affinity and epitopic specificities to develop a two-site immunoassay of rHV2. The mAb concentrations, incubation time, and temperature were optimized. The immunoassay has a detection limit for rHV2 of 45 ng/L in plasma and 30 ng/L in urine. The reactiv...

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