BACKGROUND In modern biotechnology, there is a need for pausing cell lines by cold storage to adapt large-scale cell cultures to the variable demand for their products. We compared various cell culture media/solutions for cold storage of Vero-B4 kidney cells, a cell line widely used in biotechnology. RESULTS Cold storage in RPMI 1640 medium, a recommended cell culture medium for Vero-B4 cells...

Effects of divalent cations on the regulation of glucose transport and cAMP phosphodiesterase in isolated rat epididymal adipocytes were studied. EDTA (5 mM) moderately inhibited the binding of insulin to adipocytes in Krebs-Henseleit Hepes buffer. In the same buffer, A-23187 (an ionophore specific for divalent cations; 50 microM) plus EDTA (5 mM) almost completely blocked the insulin- or hydro...

We have modified the apparatus for isolated rat liver perfusion (IPRL) in order to be able to perform two perfusions simultaneously. In addition, we studied the quality and stability of livers by comparison of five different perfusates: Blood (Group A), Original Krebs Henseleit buffer (Group B), Krebs buffer with glucose (Group C) or bovine serum albumin (BSA) added, (Group D). In a last group ...

Effects of divalent cations on the regulation of glucose transport and cAMP phosphodiesterase in isolated rat epididymal adipocytes were studied. EDTA (5 mM) moderately inhibited the binding of insulin to adipocytes in Krebs-Henseleit Hepes buffer. In the same buffer, A-23187 (an ionophore specific for divalent cations; 50 p ~ ) plus EDTA (5 mM) almost completely blocked the insulinor hydrogen ...

T-2 toxin, a trichothecene mycotoxin, is considered to be one of the most toxic compounds that are produced by molds, particularly the Fusarium species. Fusarium species have been recognized as a great agricultural problem. They occur worldwide on a variety of plant hosts and cereal grains. The aim of this study was to investigate T-2 toxin-induced liver injury using in situ perfused rat liver....

( a ) Microsomes were incubated with [ 'Tlserine (2 mM) in 40 mM-Hepes buffer, pH 7.4, containing 0.25 M-sucrose and 1 mM-CaCI,. After each incubation time the microsomes were treated with TNBS and the lipids extracted and analysed (Hutson & Hig ins, 1985). ( h ) Hepatocytes were incubated with 2 mwj"C7serine in Krebs-Henseleit buffer. The cells and medium were separated by centrifugation and t...

Activation of beta-adrenergic receptors with isoproterenol fails to stimulate prostaglandin synthesis in the isolated rabbit heart perfused with Ca2+-free Krebs-Henseleit buffer. This lack of response could be due to reduced isoproterenol-stimulated liberation of arachidonic acid, reduced cyclooxygenase activity during Ca2+-free perfusion, or both. To test these hypotheses, we have examined the...

Iron and copper toxicity has been presumed to involve the formation of hydroxyl radical ( . OH) from H2O2 in the Fenton reaction. The aim of this study was to verify that Fe 2+ -O2 and Cu + -O2 chemistry is capable of generating . OH in the quasi physiological environment of Krebs-Henseleit buffer (KH), and to compare the ability of the Fe 2+ -O2 system and of the Fenton system (Fe 2+ + H2O2) t...

Tetraethylammonium (TEA) and amantadine are two organic cations that are secreted by the kidney. It appears that each cation may characterize distinct renal tubule organic cation transport pathways. To test this hypothesis, we investigated the renal proximal and distal tubule energy-dependent transport properties of TEA and amantadine. Isolated tubules were incubated at 25 degrees C in bicarbon...