نتایج جستجو برای: hbfgf
تعداد نتایج: 13 فیلتر نتایج به سال:
AIM To obtain high-level expression of nonfusion recombinant human basic fibroblast growth factor (rhbFGF). METHODS hbFGF cDNA was prepared from the total RNA of embryonic brain tissue. As a template, the obtained gene was used to clone nonfusion rhbFGF. New primers were employed to alter the translation initiation region (TIR) and reduce the G+C content through nucleotide change. Using pET-3...
Embryonic stem cells (ESCs) are originally derived from the ICM of blastocysts and are characterized by their ability to self-renew and their pluripotencies. Only a few reports have been published on ESC isolations and line establishment in animals, even fewer in horses. However, it is still important to isolate equine ESCs for animal biotechnology and therapeutic applications. In the present s...
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