Background: Biotechnologic methods are common for secondary metabolites production from the plants and other sources in pharmaceutical sciences. Hairy root cell lines as the biotechnologic method have been used for in vitro production of major plant metabolites. Objective: In this study, hairy roots of Hypericum Perforatum have been prepared using the seeds and bio transformed by bacteria. Fin...

Background and objectives: Hyoscyamus reticulatus contains two distinguished tropane alkaloids, hyoscyamineand scopolamine and both of the compounds possesspotential acute or chronic toxicity. In the present study, a simple and efficient transformation system was established for in vitro hairy roots induction in Hyoscyamus reticulatus. M...

Background & Aim:Oxidative stresses caused by free radicals are known to promote many diseases such as inflammation and cancer; therefore, any plant product that can inhibit free radical production can play an important role in preventing diseases. We aimed to compare the fracture rate and the scavenging activity of free radicals, and the antioxidant composition of rol...

agrobacterium rhizogenes causes hairy root disease in plants. these hairy roots are ge-netically stable and grow rapidly. transformed hairy roots of hyoscyamus muticus in-duced by the bacterium can produce tropane alkaloids in trace amounts of intact plant tissues. in this research, in order to compare growth and biosynthetic stability of hairy roots with wild type ones, leaf and nodal segments...

it is an increased interest in the therapeutic potential of cannabis sativa l. (marijuana) for treatment of multiple sclerosis and hiv neuropathy. because of limitation in cultivation of this plant, an efficient hairy root induction system for cannabis sativa l. was developed in the present study. agrobacterium rhizogenes mediated transformation performed by two different co-cultivation mediums...

in order to evaluate quantity and quality hairy vetch (vicia villosa l.) yield in the pure and mixed cropping with barley (hordeum vulgare l.) and triticale (triticosecale) the experiment was conducted at agricultural research station (khodabande) under dryland conditions during 2006 -2008. field experiments were arranged in a randomized complete blocks design with 9 treatments in four replicat...

Hairy root culture is an effective method to produce secondary metabolites. In fact, hairy roots are genetically and biologically stable and also they are able to produce metabolites without hormones. Chicory (Cichorium intybus L.) is one of the most important medicinal plants with valuable medicinal compounds. In this research, hairy roots were induced by different Agrobacterium rhizogenes str...

Agrobacterium rhizogenes (pRi), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species. In our study four bacterial strains AR15834, A4, 9435 and C318 and three explants types leaf, stems and roots, were examined. Hairy roots were induced from roots, stems and leaf explants. The highest transformation efficiency of 77% was achieved by usi...

Induced hairy roots by Agrobacterium rhizogenes are suitable organs for the production of secondary metabolites, due to the stability and high production of roots without phyhormones in a short time. Fenugreek (Trigonella foenum– graecum L.) is one of the most important medicinal plants with valuable medicinal compounds. For optimizing the hairy root induction of T. foenum-graecum L., six strai...

Members of the widely conserved Hairy/Enhancer of split family of basic Helix-Loop-Helix repressors are essential for proper Drosophila and vertebrate development and are misregulated in many cancers. While a major step forward in understanding the molecular mechanism(s) surrounding Hairy-mediated repression was made with the identification of Groucho, Drosophila C-terminal binding protein (dCt...