The objectives of this study were to determine whether intestinal viscosity caused by mixed linked barley beta-glucan depresses ileal nutrient digestibility and digestive enzyme activities and to determine the interaction of intestinal viscosity, digestive enzyme activities and ileal nutrient digestibility in different ages of poultry. In Experiments 1 and 2, 1-d-old broiler chicks and 1-y-old ...

KA-prep, a culture filtrate of Bacillus circulans KA-304 grown on a cell-wall preparation of Schizophyllum commune, has an activity to form protoplasts from S. commune mycelia. alpha-1,3-Glucanase, which was isolated from an ammonium sulfate fraction of 0-30% saturation of KA-prep, gave the protoplast-forming activity to an ammonium sulfate fraction of 30-50% saturation of KA-prep, which contai...

Glucanase enzyme isolated from Trichoderma isolates were purified to homogeneity using ammonium persulphate precipitation and Fast Protein Liquid Chromatography. Purity of the isolated enzyme was confirmed by SDS-PAGE. Enzymatic properties such as effect of temperature, pH, SDS and EDTA were determined. Molecular weight of Trichoderma isolates was found to be around 55 kDa. The optimum temperat...

An experiment was conducted to determine the effect of graded levels of β-glucanase supplementation to barley (Hordeum vulgare)-based diets on the digestibility of DM, GE, N, and AA for growing-finishing pigs. Eight pigs (initial BW: 53.3 ± 3.2 kg) were each fitted with a simple T-cannula at the distal ileum and allotted to a replicated 4 × 4 Latin square design with 4 diets and 4 periods in ea...

Beta-1,3-glucanases are enzymes that hydrolyze beta-1,3-glucans, and they essential for the metabolism of seaweed, plants fungi. These also participate in digestion herbivore fungivore animals. Because importance these enzymes in insects, beta-1,3-glucanase inhibitors may be used development new control strategies against agricultural pests disease vectors. Beta-1,3-glucanase inhibito...

The beta-glucanase gene (bgl) from Bacillus amyloliquefaciens was expressed in E. coli CSH 55 under the control of the PR promoter of phage lambda that is repressed by the thermosensitive repressor C1857. Production of beta-glucanase was drastically stimulated by a temperature shift to 42 degrees C. This overexpression of the bgl gene (about 20% of the total cellular protein) led to an almost c...

β-1,3:1,4-Glucan is a major cell wall component accumulating in endosperm and young tissues in grasses. The mixed linkage glucan is a linear polysaccharide mainly consisting of cellotriosyl and cellotetraosyl units linked through single β-1,3-glucosidic linkages, but it also contains minor structures such as cellobiosyl units. In this study, we examined the action of an endo-β-1,3(4)-glucanase ...

Infection of immature pea pods with Fusarium solani f.sp. phaseoli (a non-pathogen of peas) or f.sp. pisi (a pea pathogen) resulted in induction of chitinase and beta-1,3-glucanase. Within 30 hours, activities of the two enzymes increased 9-fold and 4-fold, respectively. Chitinase and beta-1,3-glucanase were also induced by autoclaved spores of the two F. solani strains and by the known elicito...

Extracellular α-(1 → 3)-glucanase (mutanase, EC 3.2.1.84) produced by Trichoderma harzianum CCM F-340 was purified to homogeneity by ultrafiltration followed by ion exchange and hydrophobic interaction chromatography, and final chromatofocusing. The enzyme was recovered with an 18.4-fold increase in specific activity and a yield of 4.3%. Some properties of the α-(1 → 3)-glucanase were investiga...

Twelve isolates of Trichoderma spp. isolated from tobacco rhizosphere were evaluated for their ability to produce chitinase and β-1,3-glucanase extracellular hydrolytic enzymes. Isolates ThJt1 and TvHt2, out of 12 isolates, produced maximum activities of chitinase and β-1,3-glucanase, respectively. In vitro production of chitinase and β-1,3-glucanase by isolates ThJt1 and TvHt2 was tested under...