Abstract Plant breeding relies on the presence of genetic variation, which is generated by a random process mutagenesis that acts existing gene pools. This variation then recombined into new forms at frequencies impacted local euchromatin and heterochromatin environment. The result lottery where plant breeders face increasingly low odds generating “winning” genotype. Genome editing tools enable...

Programmable sequence-specific genome editing agents such as CRISPR-Cas9 have greatly advanced our ability to manipulate the human genome. Although canonical forms of genome-editing agents and programmable transcriptional regulators are constitutively active, precise temporal and spatial control over genome editing and transcriptional regulation activities would enable the more selective and po...

Chrysanthemum morifolium is one of the most popular ornamental plants in world. However, as C. a segmental hexaploid, self-incompatible, and has sizable heterologous genome, it difficult to modify its trait systematically. Genome editing technology attractive methods for modifying traits For commercial use genetically modified morifolium, rigorous stabilization quality essential. This stability...