background and objectives: a new real- time pcr protocol for the detection of trichomonas vaginalis in pooled urine samples has been optimized and validated. materials and methods: the amplification protocol, targeting a 2kb repeated gene in the t. vaginalis genome, was optimized by varying pcr parameters. as a reference method, a real-time pcr protocol targeting the beta-tubulin gene (y. versl...

introduction: beta thalassemias are a heterogenous group of autosomal recessive disorders, characterized by reduced or absent production of the b-globin chain by the affected allele. transplantation of allogenic hematopoietic stem cells (hsc) is a curative approach but this therapeutic option is not available to the majority of patients. transplantation of genetically corrected autologous hsc i...

the present study , was an attempt to develop galactosylated albumin nanoparticles of simvastatin for treatment of hypercholesterolemia. by developing the galactosylated nanoparticulated delivery the required action of drug at the target site at liver can be provided. the advantage of targeting helps to reduce the systemic side effects which may be occur due to the distribution of the drug to t...

Abstract Gene targeting is useful to isolate strains with mutations in a gene of interest for efficient breeding. In this study, we generated msh4 or mer3 single-gene disruptant monokaryons using Pleurotus ostreatus Δ ku80 strain targeting. Dikaryons P. ×Δ were isolated via backcrosses, and the number basidiospores produced was measured. The fell by an average 1/13.7 dikaryons versus + dikaryon...