DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

acute lymphoblastic leukemia(all) is due to early stage arrest of lymphoblast development. the translocation of philadelphia (ph) chromosome occurs as a result of the bcr-abl fusion gene, which constitutively produced activated tyrosine kinase. this gene fusion is an important indicator for prognosis in all and is associated with poor overall survival and remission duration. bcr-abl could inter...

dna coding for the core antigen from hepatitis b virus (hbcag) was amplified, cloned and propagated in e. coli. the core protein was expressed in e. coli and the product was readily detected by western blot. this protein can be used as a diagnostic material in serum screening tests. to increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

objective(s):to culture thein vitro mouse embryonic stem cells (mescs) and to direct their  differentiation to germ-line cells; in present study we used a vector backbone containing the fusion construct stra8-egfp to select differentiated es cells that entered meiosis.  retinoic acid was used to differentiate embryonic stem cells to germ cells. materials and methods: a fragment of stra8 gene pr...

with advent and development of dna recombinant technology and advantages of p. pastoris expression system, fusion (f) protein of pprv expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against ppr disease. in this study, f gene of pprv nigeria 75/1 strain (1637 bp) was amplified using rt-pcr and purified. it was then clon...

  With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cl...

background: the purpose of this study was to clone, express, and purify a novel multidomain fusion protein of micobacterium tuberculosis (mtb) in a prokaryotic system. methods: an hspx/esxs gene construct was synthesized and ligated into a pgh plasmid, e. coli top10 cells were transformed, and the vector was purified. the vector containing the construct and pet-21b (+) plasmid were digested wit...

Background: The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system. Methods: An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the vector was purified. The vector containing the construct and pET-21b (+) plasmid were digested ...

background: parathyroid hormone (pth) secreted by parathyroid glands regulates the metabolism of calcium and phosphorus in bone and kidney. thereby, it can stimulate bone formation, and is a promising agent in the treatment of osteoporosis. mature form of pth consists of 84 amino acids; however, the first 34 residues of pth cover the majority of hormonal action. methods: in this study, the fusi...

background and aims: since the produced recombinant proteins by molecular genetics techniques commonly have some limitations in the application, chimeric protein are introduced. chimeric proteins have found widespread application for the study of protein folding, structure stability, function and immunogenisity. methods: according to the known immunomodulatory effect and structure of hsp70 mole...