نتایج جستجو برای: fungal dna

تعداد نتایج: 556901  

2013
Eiko E. Kuramae Erik Verbruggen Remy Hillekens Mattias de Hollander Wilfred F. M. Röling Marcel G. A. van der Heijden George A. Kowalchuk

We assessed soil fungal diversity and community structure at two sampling times (t1 = 47 days and t2 = 104 days of plant age) in pots associated with four maize cultivars, including two genetically modified (GM) cultivars by high-throughput pyrosequencing of the 18S rRNA gene using DNA and RNA templates. We detected no significant differences in soil fungal diversity and community structure ass...

Journal: :The Indian journal of medical research 2006
Bansidhar Tarai A Gupta P Ray M R Shivaprakash A Chakrabarti

BACKGROUND AND OBJECTIVES Fungal endophthalmitis is a devastating form of ocular infection and postoperative endophthalmitis following cataract surgery is the commonest form of such infection. Early diagnosis is very important for effective management of these patients. As conventional techniques take longer time and lacks sensitivity, polymerase chain reaction (PCR) for detection of fungal DNA...

2014
Mikio Hirotsu Akihito Shiozawa Noritsugu Ono Masato Miwa Ken Kikuchi Katsuhisa Ikeda

OBJECTIVES/HYPOTHESIS The role of fungi in chronic rhinosinusitis (CRS) is still controversial. The present study was conducted to detect and identify fungal species from the nasal polyp tissues of eosinophilic and noneosinophilic CRS, and to determine the role of fungal antigens in cytokine production. STUDY DESIGN Prospective study. METHODS Thirty-five specimens of nasal polyps were colle...

Journal: :بینا 0
محمود نجابت m nejabat شیراز- خیابان پوستچی- مرکز تحقیقات چشم پزشکی پوستچی محمدرضا خلیلی mr khalili شیراز- خیابان پوستچی- مرکز تحقیقات چشم پزشکی پوستچی پریسا بدیعی p badiei شیراز- خیابان پوستچی- مرکز تحقیقات چشم پزشکی پوستچی فاطمه کشاورز فضل f keshavarz fazl شیراز- خیابان پوستچی- مرکز تحقیقات چشم پزشکی پوستچی عبدالوهاب البرزی a alborzi شیراز- خیابان پوستچی- مرکز تحقیقات چشم پزشکی پوستچی اطهر شادمانی a shadmani شیراز- خیابان پوستچی- مرکز تحقیقات چشم پزشکی پوستچی

purpose: to compare the value of conventional laboratory methods and polymerase chain reaction (pcr) in the diagnosis of fungal keratitis. methods: this cross sectional study was conducted at khalili hospital, shiraz, iran. samples were taken from thirty-eight patients with findings suspicious for fungal keratitis. corneal scrapings were used for gram, giemsa and koh stains, culture and pcr ana...

Journal: :Genetics and molecular research : GMR 2014
T Lech J Syguła-Cholewinska J Szostak-Kot

DNA isolation is a crucial step of conducting genetic studies in any organism. However, this process is quite difficult when studying fungi because of the need to damage the fungal cell walls of specific structures. In this study, we developed a method for the rapid and efficient isolation of fungal DNA based on simultaneous mechanical and enzymatic cell wall degradation. There are several typi...

2015
Isabella Børja Gry Alfredsen Tore Filbakk Carl Gunnar Fossdal

The demand for bioenergy caused an increased use of logging residues, branches and treetops that were previously left on the ground after harvesting. Residues are stored outdoors in piles and it is unclear to what extent fungi transform this material. Our objective was to quantify the amount of wood degrading fungi during storage using quantitative real-time PCR (qPCR) to detect basidiomycetous...

Journal: :Journal of clinical microbiology 2016
S Czurda S Smelik S Preuner-Stix F Nogueira T Lion

Nucleic acid amplification techniques permitting sensitive and rapid screening in patients at risk for invasive fungal infections are an important addition to conventional fungal diagnostic methods. However, contamination with fungal DNA may be a serious threat to the validity of fungal amplification-based assays. Besides rigorous handling procedures to avoid false-positive test results from ex...

RNA processing is essential factor for synthesis of functional and structural proteins in eukaryote cells. In eukaryote organisms it will be initiated with transcription of DNA in nucleolus and terminated to mRNA translation in cytoplasm, finally mRNA degraded. Protein synthesis followed as different steps, includes 5' capping, poly adenylating, processing and transferring from nucleolus to cyt...

2013
GUIHONG CAI

Cai, G. 2013. Fungal DNA, Mould, Dampness and Allergens in Schools and Day Care Centers and Respiratory Health. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 942. 85 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-8788-1. Day care centers and schools are important environments for children, but few epidemiological studies exist from these env...

Journal: :Acta biochimica Polonica 2010
Urszula Nawrot Katarzyna Wlodarczyk Magdalena Wrobel Anita Wasik Tadeusz Dobosz

The aim of this study was to compare the efficiency of DNA extraction from water as well as from blood samples spiked with A. fumigatus spores, using selected commercial kits. Extraction of DNA according to manufacturer's protocols was preceded by blood cells lysis and disruption of fungal cells by enzymatic digestion or bead beating. The efficiency of DNA extraction was measured by PCR using A...

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