Carnosic acid (CA), carnosol (CL) and rosmarinic (RA), components of the herb rosemary, reportedly exert favorable metabolic actions. This study showed that both CA CL, but not RA, induce significant phosphorylation AMP-dependent kinase (AMPK) its downstream acetyl-CoA carboxylase 1 (ACC1) in HepG2 hepatoma cells. Glucose-6-phosphatase (G6PC) phosphoenolpyruvate carboxykinase (PCK1), rate-limit...

1. Forskolin is often used to activate adenylate cyclase in studies relating adenosine 3',5'-cyclic monophosphate (cAMP) to the modulation of membrane current. There is growing concern, however, that some actions of forskolin are independent of cAMP. With the use of two-electrode voltage-clamp techniques, we compared the effects of analogues of cAMP to the effects of forskolin on K+ currents in...

Cells with the morphological properties of endothelial cells were cultured from the rat aorta. The cultured cells accumulated 45Ca2+ from the medium in a manner which was stimulated by forskolin and by 8-bromo-cyclic AMP. Pretreating the cultures for 20 h with forskolin diminished forskolin-dependent Ca2+-uptake activity. Adenylate cyclase activity of cultured cell homogenates was stimulated by...

The homologous and heterologous desensitization of rat Leydig-tumour-cell adenylate cyclase induced by lutropin (LH) was characterized with the aid of forskolin and cholera toxin. Forskolin stimulated cyclic AMP production in a dose-dependent manner, with linear kinetics up to 2 h. Forskolin also potentiated the action ofLH on cyclicAMP production, but was only additive with cholera toxin. Prei...

Forskolin potently activates all cloned mammalian adenylyl cyclases except type IX by interacting with two homologous cytoplasmic domains (C1 and C2) that form the catalytic core. A mutational analysis of the IIC2 protein (C2 domain from type II adenylyl cyclase) and forskolin analogs suggests that Ser942 interacts with the 7-acetyl group of forskolin. The C1/C2 complex has only one forskolin, ...

Forskolin is a unique structurally complex labdane-type diterpenoid used in the treatment of glaucoma and heart failure based on its activity as a cyclic AMP booster. Commercial production of forskolin relies exclusively on extraction from its only known natural source, the plant Coleus forskohlii, in which forskolin accumulates in the root cork. Here, we report the discovery of five cytochrome...

To visualize the adenylate cyclase (AC)-related second messenger system, [11C]forskolin, [11C]1-acetyl-7-deacetylforskolin, [11C]1,9-dideoxyforskolin and [11C]1-deoxyforskolin were synthesized by acetylation of the respective deacetyl-precursors using [11C]acetylchloride and dimethylaminopyridine. The radiochemical yield of [11C]forskolin, [11C]1-acetyl-7-deacetylforskolin, [11C]1,9-dideoxyfors...

Medicinal herbs are moving from fringe to mainstream use with a greater number of people seeking remedies.Forskolin, is a diterpene, the main active ingredient in the ayurvedic herb Coleus forskohliihas been used in India since prehistoric times in ayurvedic traditional medicine.Coleus forskohliiis a perennial aromatic herb found in subtropical regions of India, Nepal and Srilanka.Forskolin act...

introduction: the aim of this study was to evaluate the changes in camp and some other cellular ingredients following adenyl cyclase activation in paragigantocellularis (pgi) neurons of morphine dependent and withdrawn rats. material and methods: the 3.5 mm thick brainstem slice prepared from pgi area (-10.04 to -13.24 from bregma) of control and morphine dependent rats (n=5 in each group). wer...