نتایج جستجو برای: formaldehyde fixative
تعداد نتایج: 12052 فیلتر نتایج به سال:
BACKGROUND Formaldehyde crosslinking is in widespread use as a biological fixative for microscopy and molecular biology. An assumption behind its use is that most biologically meaningful interactions are preserved by crosslinking, but the minimum length of time required for an interaction to become fixed has not been determined. METHODOLOGY Using a unique series of mutations in the DNA bindin...
On 10 June 2011, the US National Toxicology Program described formaldehyde as "known to be a human carcinogen". However, formaldehyde is not just a human carcinogen but the cause of other many hazards; respiratory distress, red eyes, etc. Occupational health authorities throughout the world are therefore likely to increase the strictness of regulations for the use of formaldehyde within anatomi...
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy Katharina N. Richter, Natalia H. Revelo, Katharina J. Seitz, Martin S. Helm, Deblina Sarkar, Rebecca S. Saleeb, Elisa D’Este, Jessica Eberle, Eva Wagner, Christian Vogl, Diana F. Lazaro, Frank Richter, Javier Coy-Vergara, Giovanna Coceano, Edward S Boyden, Rory R. Duncan, Stefan W. Hell, Marcel ...
BACKGROUND Formaldehyde (HCHO) is a gas (available as a 37% concentrated solution, stabilized with methanol). The 10% dilution (approximately 4% formaldehyde) has been used as a fixative since the end of the 19th century. Alternative fixatives are also commercially available or may be prepared in-house in laboratories. Statements by the IARC, along with other USA agencies (CalEPA, RoC/NTP) on t...
Using indirect immunofluorescence, indirect immunoperoxidase, and unlabelled antibody enzyme techniques, gastrin, pancreatic glucagon, insulin, and somatostatin were localised in sections of both wax- and resin-embedded tissues that had been fixed in a buffered formalin solution. Ultrastructural preservation of the resin-embedded samples was also adequate for combined electron microscopy and li...
Formalin is widely used in industry and in medicine (as tissue fixative and disinfectant).It contains reactive molecules which have been known for its cytotoxic effects. To evaluate the effect of formalin exposure on the testicular tissue and sperm parameter from neonatal period through physical and sexual maturity, 28 male Wister rats were assigned into two equal test and control groups. The t...
If fixation is not adequate, the other processes that follow such as dehydration, clearing, infiltration, embedding, microtomy and staining, will also be inadequate. A poorly processed tissue make it difficult for Pathologist/Histoscientist to render a proper diagnosis. This article aimed at elucidating more on fixatives process in histopathology. The information presented this review was gathe...
Conventional methods to preserve adult nematodes for taxonomic purposes involve the use of fixative or clearing solutions (alcohol, formaldehyde, AFA and lactophenol), which cause morphological alterations and are toxic. The aim of this study is to propose an alternative method based on glycerol-cryopreservation of nematodes for their subsequent identification. Adults of trichostrongylid nemato...
Heavy metal impregnation of specimens for electron microscopy offers a means for differential "staining" of various tissue components and cellular constituents. Of many silver impregnation methods, one of the most useful is Gomori's silver methenamine (1) after periodic acid oxidation (PA-SM) (2, 3). Several authors have applied silver impregnation to electron microscopy (4-7), mainly for the d...
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