Ex vitro strawberry plantlets from micropropagation and coinoculated with arbuscular mycorrhizal fungi (AMF) biochar can provide beneficial health effects. In the present study, we evaluated effects of different proportions in presence absence AMF on production secondary metabolites leaves roots during ex acclimatization. Additionally, enzymatic activity substrate enriched was analyzed. The exp...

This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA3 or 0.2 µM NAA, sucrose at 15 or 30 g L-1, in test tubes which al...

In order to improve the shoot proliferation rate of ‘Dwarf Cavendish’ banana shoot tip cultures, polyethylene glycol (PEG6000) was employed at concentrations of 2, 4, and 6% (w/v). The medium without PEG was considered as negative control. An increasing of shoot proliferation was achieved in MS medium containing 2% PEG. Furthermore, application of 2% PEG significantly increased the shoot dry we...

Conventional in vitro propagation may affect normal morphophysiological characteristics and survival during the acclimatization of commercial plants, such as Etlingera Elatior (Jack) R.M. Smith. CO2 enrichment, gas-permeable membranes, reduced carbohydrate concentration culture media can be used to mitigate these effects. Here, we aimed determine impact photomixotrophic photoautotrophic growth ...

Plant tissue culture refers to growing and multiplication of cells, tissues and organs of plants on defined solid or liquid media under aseptic and controlled environment. Micropropagation allows rapid production of high quality, disease-free and uniform planting material. The micropropagation of high quality planting materials of ornamentals, and forest and fruit trees has created new opportun...

This study describes the in vitro propagation and ex acclimatization of Philodendron erubescens pink princess, one most popular ornamental variegated foliage plants. For shoot proliferation, protocorm-like bodies princess were cultured on solid Murashige Skoog (MS) media supplemented with 6-benzylaminopurine (BAP) 1-naphthaleneacetic acid (NAA) at different concentrations. The results revealed ...

Successful in vitro propagation through ex vitro rooting mechanism has been achieved in Piper longum by nodal shoot segment cultures. Shoot tip explants were less proliferative compared to the nodal meristems. The explants were sterilized using 0.1% HgCl2 and cultured on Murashige and Skoog (MS) medium with various concentrations of growth regulators. MS medium supplemented with 1.0 mg/L 6-benz...