The possibility of replacing the rabbit pyrogen test by the Limulus Amebocyte lysate (LAL) test, as a final release test for Large Volume Parenterals (LVPs) was investigated. The sensitivity and specificity of the LAL test and rabbit pyrogen assay were studied by means of artificially contaminated parenterals. Various LVPs (mostly containing electrolytes) were spiked with 0.5 and 5 EUlml o...

haemorrhagic septicaemia (hs) vaccine which is prepared in razi institute is used in endemic areas ofiran. aluminum-hydroxide gel was used as adjuvant for preparing this vaccine. post-vaccinal shock reactionswere the main complaint after use of this vaccine. in a previous study, we could improve the vaccine byalum-precipitation pasteurella multocida cells and removing the liquid phase. in this ...

This work was aimed to improve the efficacy of tacrolimus in treatment endotoxin-induced uveitis (EIU) using propylene glycol modified lipid vesicles termed as proglycosome nano-vesicles (PNVs). PNVs were prepared by film hydration method. Experimental rabbit eye induced an intravitreal injection 20 ?L endotoxin solution containing 100 ng lipopolysaccharide endotoxin. In vivo determined studyin...

Host proinflammatory responses to minute amounts of endotoxins derived from many Gram-negative bacteria require the interaction of lipopolysaccharide-binding protein (LBP), CD14, Toll-like receptor 4 (TLR4) and MD-2. Optimal sensitivity to endotoxin requires an ordered series of endotoxin-protein and protein-protein interactions. At substoichiometric concentrations, LBP facilitates delivery of ...

The importance of free amino acids in the processes vital activity normal and pathological conditions is well known. At same time, there are no data on role blood plasma valine formation thyroid status, prooxidant-antioxidant state, development hyperthermia caused by bacterial endotoxin, although its participation these quite natural, since an inhibitor arginase, which affects level arginine ac...

Graphene and titanium carbide MXene variants significantly reduced bacterial endotoxin induced THP-1 monocyte IL-8, IL-6 TNF-α cytokine production.

Chylomicrons prevent endotoxin toxicity and increase endotoxin uptake by hepatocytes. As a consequence, less endotoxin is available to activate macrophages, thereby reducing tumor necrosis factor secretion. To determine whether the chylomicron-mediated increase in hepatocellular uptake of endotoxin results in increased endotoxin excretion into bile, we examined bile after endotoxin administrati...

background: chemical and microbial quality of water used in hemodialysis play key roles in a number of dialysis-related complications. in order to avoid the complications and to guarantee safety and health of patients therefore, vigorous control of water quality is essential. the objective of present study was to investigate the chemical and bacteriological characteristics of water used in dial...

Exposure to elevated levels of endotoxin in family-room dust was previously observed to be significantly associated with increased wheeze in the first year of life among a cohort of 404 children in the Boston, Massachusetts, metropolitan area. However, it is likely that family-room dust endotoxin was a surrogate for airborne endotoxin exposure. Therefore, a related substudy characterized the re...