Prunus necrotic ringspot virus (PNRSV) and cherry A (CVA) are two viruses that mainly infect plants of the genus Prunus. Full-length sequences these viruses, collected in Czech Republic from cerasifera plants, were obtained via HTS sequencing. Phylogenetic analyses based on NJ method Splitstree tools showed PNRSV isolate (ON088600-ON088602) is a divergent other molecular groups, sharing less th...

The movement protein (MP) of Prunus necrotic ringspot virus (PNRSV) is required for viral transport. Previous analysis with MPs of other members of the family Bromoviridae has shown that the C-terminal part of these MPs plays a critical role in the interaction with the cognate coat protein (CP) and in cell-to-cell transport. Bimolecular fluorescence complementation and overlay analysis confirm ...

The aim of this work was to follow Prunus necrotic ringspot virus (PNRSV) infection in apricot reproductive tissues and transmission of the virus to the next generation. For this, an analysis of viral distribution in apricot reproductive organs was carried out at different developmental stages. PNRSV was detected in reproductive tissues during gametogenesis. The virus was always present in the ...

abstract in october 2009, in one of the alstroemeria (alatroemeria sp.) greenhouses, located in khorasan razavi province, tospovirus-like symptoms (necrosis) were observed on stems, petioles, flowers and leaves of some plants. sap extracts from these plants were mechanically inoculated to indicator plants. necrotic local lesions were observed on petunia hybrida and chenopodium quinoa. necrotic ...

The distribution of Ilarvirus species populations amongst 61 Australian Prunus trees was determined by next generation sequencing (NGS) of amplicons generated using a genus-based generic RT-PCR targeting a conserved region of the Ilarvirus RNA2 component that encodes the RNA dependent RNA polymerase (RdRp) gene. Presence of Ilarvirus sequences in each positive sample was further validated by Sa...

The movement protein (MP) of Prunus necrotic ringspot virus (PNRSV) is involved in intercellular virus transport. In this study, putative RNA-binding properties of the PNRSV MP were studied. The PNRSV MP was produced in Escherichia coli using an expression vector. Electrophoretic mobility shift assays (EMSAs) using DIG-labelled riboprobes demonstrated that PNRSV MP bound ssRNA cooperatively wit...

We conducted a survey of the phytosanitary status Prunus germplasm collection in Nikita Botanical Gardens, Yalta, Russia. The virome plants displaying virus-like symptoms was studied using Illumina MiSeq high-throughput sequencing. Reads related to prunus necrotic ringspot virus (PNRSV), prune dwarf (PDV), and ourmia-like 1 (OuLV1) were generated number samples. Near complete genomes two diverg...

Background and Amis: Serological assay is considered as one of the best choices for conducting large number of infection tests. Recombinant DNA technology has been used for expression of virus coat protein (CP) gene in prokaryotic bacterial cells such as Escherichia coli and the recombinant CP (rCP) is used as immunogen in antibody production. Heterologous CP protein expression and purification...

The reaction of the transgenic plum pox virus (PPV) resistant C-S plum was studied under conditions of co-infection with Prunus necrotic ringspot virus (PNRSV) and PPV as may he encountered in the field. C-S and controls were inoculated with PPV and PNRSV following several co-infection schemes. The results of this preliminary study showed that, as expected, the transgenic clones, including C-S ...