We have studied the influence of verapamil hydrochloride on the in vitro and in vivo effects of daunorubicin in Ehrlich ascites carcinoma. Daunorubicin-sensitive tumor was rendered resistant to daunorubicin by the continuous treatment of sequential generations of tumor-bearing BALB/c mice. The ability of daunorubicin to inhibit [(3)H]uridine and [(3)H]thymidine incorporation and the effect of d...

The aim of this study is to investigate the antitumor activity and possible molecular mechanism of Phenethyl isothiocyanate (PEITC) against Ehrlich ascites carcinoma in-vivo and in-vitro.In-vivo, ascetic fluid volume, body weight, serum malondialdehyde (MDA) level and total antioxidant capacity (TAC) were determined using Ehrlich ascites carcinoma (EAC) bearing mice. In-vitro, MTT assay was use...

the antitumor activity of ethanol extract of prosopis glandulosa torr. (epg) was evaluated against ehrlich ascites carcinoma (eac) tumor model in swiss albino mice on dose dependent manner. the activity was assessed using survival time, average increase in body weight, hematological parameters and solid tumor volume. oral administration of epg at the dose of 100, 200 and 400 mg/kg, significantl...

The aim of this study is to investigate the antitumor activity and possible molecular mechanism of Phenethyl isothiocyanate (PEITC) against Ehrlich ascites carcinoma in-vivo and in-vitro.In-vivo, ascetic fluid volume, body weight, serum malondialdehyde (MDA) level and total antioxidant capacity (TAC) were determined using Ehrlich ascites carcinoma (EAC) bearing mice. In-vitro, MTT assay was use...

Azaserine has been shown to be a potent in hibitor of de novo purine synthesis in both solid tumors (1, 13) and ascites cells (5, 6, 10). The duration of inhibition has been correlated with increases in the survival time of tumor-bearing hosts (7, 8). During inhibition of de novo purine synthesis by azaserine, the ascites cells maintain the ability to utilize preformed purines and pre sumably s...

Previous studies from this laboratory demonstrated that a potent inhibitor of nucleoside transport, nitrobenzylthioinosine (NBMPR), protected cultured cells against cytotoxic nucleosides (nebularine, tubercidin, and toyocamycin). NBMPR and its 5'-monophosphate (NBMPR-P) also protected mice against potentially lethal dosage of these agents. This report describes protection of mice from potential...

Fig. 1. Effect of rifamycins on RNA polymerase reactions of E. coli and Ehrlich carcinoma cells. The reaction mixture for the assay of E. coli RNA polymerase contained (0.3 ml) Tris-HCl, pH 8.0, 15 //moles; /?-mercaptoethanol, 3.6 //moles; MgCl2, 1.2 //moles; MnCl2, 0.3 //moles; ATP, GTP and UTP, 0.1 //mole each; 3H-CTP, 0.05 //moles (3,000 cpm/m/zmole); calf thymus DNA, 30 jug, enzyme, 20jug. ...

Gilvocarcin V, isolated rom a Streptomyces culture showed activity against experimental tumors such as sarcoma 180, Ehrlich carcinoma, Meth 1 fibrosarcoma, MH134 hepatoma and lymphocytic leukemia P388. In particular, 40% of treated mice survived for 60 days, after intraperitoneal administration of gilvocarcin V to mice bearing Ehrlich ascites carcinoma. But it was marginally active against B16...

The Ehrlich ascites carcinoma of mice, first described by Loewenthal and Jahn (26), has in recent years been the subject of a great many investigations. Klein et cd. have studied certain of the biological properties and the nucleic acid con tent of this tumor (15, 17), and Christensen and co-workers (7, 8) have inquired into the capacity of the tumor to concentrate amino acids. Moldave (33) has...

吉村 政弘 ・蕪城 外枝子 * This work was supported , in part, by grants from the Japan Anti-Cancer Society, the National Institute of Health, U. S. A., and the Tokyo Biochemistry Research Fund. Attempts to induce immunity against Ehrlich ascites carcinoma in inbred mice have been the subject of considerable studies in recent years (1-4). Various procedures were employed with varying degrees of success to ...