Coenzyme Q0 (Q0), a strong electrophile, is toxic to insulin-producing cells. Q0 was incubated with rat and human pancreatic islets and INS-1 insulinoma cells, and its attachment to cellular proteins was studied with Western analysis using antiserum raised against the benzoquinone ring structure of ubiquinone (anti-Q). Q0 covalently bonded to two proteins, one of 50 kDa and another of 70 kDa. B...

Optimal care for breast cancer patients undergoing aromatase inhibitor (AI) treatment is ensured when estradiol (E2) levels are adequately suppressed. To assess treatment efficacy accurately, it is important to measure the serum E2 levels using a well validated assay method with high sensitivity and specificity. This translates into the urgent need to evaluate various E2 immunoassay kits, which...

Neonatal administration of estradiol-17beta (E2-17beta) increases the nuclear DNA content in the mouse reproductive tract. Similar responses have been demonstrated for synthetic estrogens such as diethylstilbestrol. One of the questions raised regarding environmental estrogens such as organochlorines is whether they are potent enough to result in abnormal changes such as those demonstrated by b...

Bacterial strains D11, E1 and E2 isolated from petroleum-contaminated soils were found to be members of Acinetobacter genus revealed by 16S rRNA gene sequence analysis phenotypic characteristics. After incubation for 5 days, about 43%, 9% 12% total petroleum hydrocarbons crude oil degraded E2, respectively, determined GC-MS analysis. Moreover, 70% 76% single hydrocarbon hexadecane was the D11 a...

Abstract. According to Modern-Era Retrospective Research Analysis for and Applications (MERRA-2) temperature wind datasets in 2019, this study presents the global variations eastward-propagating wavenumber 1 (E1), 2 (E2), 3 (E3) 4 (E4) planetary waves (PWs) their diagnostic results polar middle atmosphere. We clearly demonstrate eastward wave modes exist during winter periods with westward back...

Cell culture-adapted laboratory strains of Sindbis virus (SB) exhibit efficient initial attachment to cell surface heparan sulfate (HS) receptors. In contrast, non-cell-adapted strains, such as the SB consensus sequence virus TR339, interact weakly with HS and cell surfaces. Regardless of their HS binding phenotype, most SB strains do not cause fatal disease in adult mice, whether inoculated su...

Estradiol (E2) facilitates the lordosis reflex that occurs in response to flank stimulation in female rats. Lordosis appears to be regulated in part by the synthesis of proteins in the ventral medial hypothalamus (VMH) that are transported to the midbrain central gray (MCG). We developed a strategy involving microinfusion of radioactive amino acids, followed by 2-dimensional gel electrophoresis...

OBJECTIVE To search for novel autoantibodies in patients with rheumatoid arthritis (RA) in an effort to better understand the processes of joint destruction in this disease. METHODS Using a modified SEREX technique and complementary DNA derived from RA synovium, serpin E2 was identified as a novel autoantigen and was analyzed by immunohistochemistry. Levels of anti-serpin E2 autoantibodies in...

El objetivo del estudio fue determinar la frecuencia y carga de ectoparásitos en branquias alevinos gamitana procedentes tres establecimientos piscícolas crianza semi-intensiva provincia Coronel Portillo, Ucayali, Perú. Se seleccionaron 60 (35-45 días edad) por cada uno los proveedores (E1, E2 E3), criados a condiciones similares densidad alimentación. evaluaron secciones capas branquiales ambo...

Intravaginal impedance (IVI) fluctuates during the goat estrous cycle. To understand which ovarian steroids are responsible for IVI changes and whether IVI variations are associated with precopulatory and copulatory behaviors, 8 ovariectomized females were assigned to 4 treatments in a 4 x 4 Latin square replicated over four 8-d periods. The treatments were as follows: progesterone plus estradi...