Background :Wide distribution and low half-life of acyclovir has led to a high dose consumption of the drug. Recent studies have shown that encapsulation of acyclovir in nano-carriers can increase effectiveness and decrease its side effects. We investigated the inhibitory effect of acyclovir loaded nano-niosomes against herpes simplex virus type-1 (HSV-1) in cell culture.   Methods : In-vitro...

Anti-drug antibodies induced by biologic therapeutics often impact drug pharmacokinetics, pharmacodynamics response, clinical efficacy, and patient safety. It is critical to assess the immunogenicity risk of potential biotherapeutics in producing neutralizing and nonneutralizing anti-drug antibodies, especially in clinical phases of drug development. Different assay methodologies have been used...

The present study has been designed to evaluate the impact of co-administration pantoprazole (PNT) with vitamin B (VTB) complex (VTB comprising VTB1, VTB6, and VTB12 in this study) on pharmacokinetic behavior. In study, HPLC-based sensitive efficient methods for simultaneous determination human plasma were developed per US-FDA bioanalytical standards. parameters PNT, also evaluated when medicin...

Fleming researchers have developed an actin-based cellular assay for drug discovery in treating Rheumatoid Arthritis (RA). The assay uses primary synovial fibroblasts (SF) isolated from the joints of proprietary arthritic transgenic mice overexpressing human TNF. The assay accurately quantifies the intracellular level of F-actin using fluorescencently-labelled phalloidin. Reduced F-actin levels...

4 Luminescence-based assay methods in drug discovery

In the present research, we tend to ready a series of novel pyrimidine-linked isoxazole derivatives (11-20). The molecular structure and elemental composition these compounds were confirmed by spectroscopic studies analysis. MABA (Microplate alamar Blue Assay) assay was employed for assessing antitubercular activity against Mycobacterium tuberculosis H37Rv strain. Among ten synthesized compound...