Cellobiose labeled either at the reducing end (4-O-beta-d-glucopyranosyl-d-[U-C]glucopyranose) or at the nonreducing end (4-O-beta-d-[U-C]glucopyranosyl-d -glucopyranose) was synthesized with Clostridium thermocellum cellobiose phosphorylase at greater than 98% purity. The radioactive cellobioses were identical to authentic cellobiose in melting point, optical rotation, isotopic dilution, and c...

One virulent phage specific to Bacillus Subtilis was isolated from soil cultivated with liquorices root in Matruh governorate, Cairo, Egypt. The B. subtilis phage isolate produced 0.13 cm diameter circular plaques; turbid and without central zone. In vitro determination of thermal inactivation point (TIP), dilution end point (DEP) and longevity in vitro (LIV) were found to be 68°C/10 min, 10 an...

The contamination of ores with wastes or materials of lower than the cut-off grade is referred to as dilution. Dilution is an undesirable phenomenon that, on one hand, reduces the product grade and, consequently, reduces the sales prices and, on the other hand, adds an extra cost to waste production. Therefore, studying and evaluating the dilution risk is important in mining, and especially in ...

The method of preparing protein-free filtrates by the use of tungstic acid, as developed and used by Folin, has proved so highly satisfactory in the determination of a number of blood constituents, that it has seemed desirable to apply it to the determination of chlorides. The procedure developed for this purpose is based upon the principle of the Volhard method;l namely, the precipitation of s...

The method of preparing protein-free filtrates by the use of tungstic acid, as developed and used by Folin, has proved so highly satisfactory in the determination of a number of blood constituents, that it has seemed desirable to apply it to the determination of chlorides. The procedure developed for this purpose is based upon the principle of the Volhard method;l namely, the precipitation of s...

The method of preparing protein-free filtrates by the use of tungstic acid, as developed and used by Folin, has proved so highly satisfactory in the determination of a number of blood constituents, that it has seemed desirable to apply it to the determination of chlorides. The procedure developed for this purpose is based upon the principle of the Volhard method;l namely, the precipitation of s...

Enzyme-linked immunosorbent assays (ELISA) were developed for quantifying cytomegalovirus (CMV) and rubella antibodies using a single serum dilution (1/800) in conjunction with a standard curve. A near linear relation was found between the logarithms of absorbance values of sera at a dilution of 1/800 and the titres as determined by an end point dilution ELISA. The reproducibility of the single...