A thin layer chromatographic-densitometric method has been developed for identification and quantitative determination of neomycin derivative with dabsyl chloride. The analysis of antibiotic was achieved on the silica gel TLC plates with fluorescent indicator with n-butanol--2-butanone--25% ammonia--water (10 : 6 : 2 : 2, v/v/v/v) as the mobile phase. The densitometric measurements were made at...

Adsorption thin layer chromatography (NP-TLC) with densitometry has been established for the identification and the quantification of acetaminophen in three leading commercial products of pharmaceutical tablets coded as brand: P1 (Product no. 1), P2 (Product no. 2), and P3 (Product no. 3). Applied chromatographic conditions have separated acetaminophen from its related substances, namely, 4-ami...

Mesterolone is a synthetic androgenic steroid indicating a weak anabolic activity. A new, simple in use, and economical TLC-densitometric method in normal phase system (NP-TLC) has been developed and validated for the identification and quantitative determination of mesterolone in bulk drug and in tablet formulation. NP-TLC analysis was performed on aluminium plates precoated with silica gel 60...

Objective: The aim of this study was to develop and validate an image analysis method for quantitative analysis of barakol content in Senna siamea leaf extract. Methods: TLC-densitometric and TLC-image analysis methods were developed, validated, and used for quantitative analysis of Senna siamea leaf extract. The results obtained by these two different quantification methods were compared by pa...

Densitometry was used for quantitative determination of arbutin (Vaccinium vitis idaeae) in leaves of cowberry collected from region of Suwalszczyzna, Poland. Arbutin was extracted using methanol. Chromatography was performed on glass TLC plates with layers of silica gel. The quantitative densitometric analysis was performed using internal standard solution method. On the base of densitometric ...

Paraproteins have been estimated by both immunochemical methods and densitometric analysis of electrophoretic strips. Correlation between the results obtained by these two methods, although generally good for assessing trends, varied between patients.