Comprehensive characterization of physicochemical properties monoclonal antibodies (mAbs) is a critical process to ensure their quality, efficacy, and safety. For this purpose, mAb analysis at different levels (bottom-up, middle-up) common approach that includes rather complex multistep sample preparation (reduction, digestion). To high performance, the development fully integrated methodologie...

The origin of the word “allergy” is in the Greek words “allos” (strange) and “ergon” (reaction), and it is a concept that was proposed in 1906. Atopic dermatitis, allergic rhinitis and other diseases that are caused by allergies have recently become major problems. The various allergens that exist in the environment are causes of these allergic diseases. There are many types of allergens, inclu...

Hybridization of nucleic acids to microarrays is a crucial step for several biological and biomedical applications. However, the poor efficiency and resulting long incubation times are major drawbacks. In addition to diffusion limitation, back-hybridization to complementary strands in solution is shown to be an important cause of the low efficiency. In this paper repeated denaturing in an integ...

Membrane and nuclear proteins of poor solubility have been separated by high resolution two-dimensional (2-D) gel electrophoresis. Isoelectric focusing with immobilized pH gradients leads to severe quantitative losses of proteins in the resulting 2-D map, although the resolution is usually high. Protein solubility could be improved by using denaturing solutions containing various detergents and...

Metals bound to proteins play essential roles in living systems. Elements such as phosphorus, selenium and iodine are commonly covalently linked to proteins while others are non-covalently complexed. Thus, the identification and characterization of the metal-protein complexes require a careful hyphenation of techniques able to separate and detect the intact binding complexes with both high reso...