it is now universally accepted that dna is the main target for damages caused by physical and chemical genotoxicants. although there are different methods to measure directly the induced dna damages but due to fast repair processes in cellular environment, most of the damages would be repaired even before sampling, therefore processed dna damages, i.e. damages left unrepaired after acting repai...

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It is now universally accepted that DNA is the main target for damages caused by physical and chemical genotoxicants. Although there are different methods to measure directly the induced DNA damages but due to fast repair processes in cellular environment, most of the damages would be repaired even before sampling, therefore processed DNA damages, i.e. damages left unrepaired after acting repai...

introduction: ionizing radiation induces various kinds of dna damage in which may lead to chromosomal aberrations (ca). inspite of growing importance in the risk assessment, the dose yield kinetics of ca and their implications for dose assessment are not well established in exposures to low level radiation. in the present study, cytochalasin-b blocked micronucleus assay and metaphase analysis w...

Background: Most of the cytogenetic dosimetry, except Premature Chromosome Condensation assay, saturates at 6 Gy. Present studies are aimed to assess feasibility of simple CB-MN assay for prescreening of high dose accidental exposures. Materials and Methods: Human peripheral blood lymphocytes from two donors were gamma irradiated to different doses ranging from 0-15Gy, cultured for 72 h. Cytoch...

Standard toxicity tests might not be fully adequate for evaluating nanomaterials since their unique features are also responsible unexpected interactions. The in vitro cytokinesis-block micronucleus (CBMN) test is recommended genotoxicity testing, but cytochalasin-B (Cyt-B) may interfere with nanoparticles (NP), leading to inaccurate results. Our objective was determine whether Cyt-B could MN i...

The aim of this study was to investigate the genotoxic effects of flunixin meglumine on mice peripheral lymphocytes by in vitro and in vivo/invitro cytokinesis block micronucleus tests (CBMN). Flunixin meglumine was used at concentrations of 25, 50 and 100 μg/mL for the in vitro assay and 50, 75 and 100 mg/kg for the in vivo/in vitro assay. Mice were treated intraperitonally twice with a 24 h i...

OBJECTIVE A previous study by our research group evaluated the levels of DNA damage using the comet assay in hemodialysis patients with type 2 diabetes mellitus. The same blood samples were also evaluated using the cytochalasin B micronucleus assay. A comparison of the results of the two assays is presented here. METHODS Whole blood samples were collected from 22 type 2 diabetes mellitus pati...

The objective of the study was to investigate the mitogenic and genotoxic effects of He:Ne laser irradiation (632.8 nm) on human peripheral lymphocytes in vitro. We used the cytokinesis-block micronucleus assay, which incorporates cytochalasin B to inhibit cytokinesis while karyokinesis proceeds normally leading to the appearance of proliferating lymphocytes as binucleated cells. Also micronucl...

The potential effect of electromagnetic fields (EMFs) emitted from video display terminals (VDTs) to elicit biological response is a major concern for the public. The software professionals are subjected to cumulative EMFs in their occupational environments. This study was undertaken to evaluate DNA damage and incidences of micronuclei in such professionals. To the best of our knowledge, the pr...