The binding reaction between carcinoembryonic antigen (CEA) and concanavalin A (Con A) has been studied. CEA, prepared from liver métastasesin patients with carcinoma of the colon, was tested against Con A for precipitation. By using the techniques of immunodiffusion and counlerelectrophoresis, a precipitate line between CEA and Con A was observed, which was different from that seen between ei...

Prions are formed of misfolded assemblies (PrP(Sc)) of the variably N-glycosylated cellular prion protein (PrP(C)). In infected species, prions replicate by seeding the conversion and polymerization of host PrP(C). Distinct prion strains can be recognized, exhibiting defined PrP(Sc) biochemical properties such as the glycotype and specific biological traits. While strain information is encoded ...

Human placenta alkaline phosphatase (HP-ALP), a glycoprotein, was stained histochemically for the purpose of examining the concanavalin A (Con A) binding sites on the cell surface. HP-ALP was bound to the cell surface by Con A. This simple method successfully detected Con A binding sites on the cell surface.

cultured lymphoblasts required physical contact with responding lymphocytes to induce transformiiation. In quantitative terms, lymphocytes incorporate as much thymidine when,mixed with irradiated cultured lymphoblasts as they (lo in response to l)hytolhenma-glutin1in. The stimulation of lymphocyte transformation by allogeneic cultured lymphoblasts did not parallel the stimulation of lymphocyte ...

Concanavalin A (Con A) concentration dependence of agglutinability of a mutant of Rous sarcoma virus which is temperature-sensitive for transformation was examined. Con A agglutinability of the virion was quantitatively expressed by measuring radioactivities of 60 to 70 S RNA extracted from Con A-agglutinated material. The mutant grown at a permissive temperature (35 degrees C) agglutinated at ...

We have demonstrated that stable Concanavalin A (Con A)-coated supramolecular nanofibers can be constructed by complexation of self-assembled mannose nanofibers with Con A. The extended multivalent Con A nanofibers bind strongly to T cell surfaces and promote T cell activation.

Peritoneal macrophages from mice injected 4 days previously with Brewer's thioglycollate medium have a pinocytic rate, in culture, of 190 ng horseradish peroxidase (HRP)/100 mug cell protein/h, compared to the rate of resident peritoneal cells of 53 ng HRP/100 mug cell protein/h. Mice injected with endotoxin or with only certain of the components of the Brewer's medium show an intermediate leve...

Binding of the chromogenic ligand p-nitrophenyl a-n-mannopyranoside to concanavalin A was studied in a stopped-flow spectrometer. Formation of the protein-ligand complex could be represented as a simple one-step process. No kinetic evidence could be obtained for a ligand-induced change in the conformation of concanavalin A, although the existence of such a conformational change was not excluded...