To explore the influence of long-non coding RNA-H19/microRNA-140-5p/Wnt1 axis on osteogenic differentiation in bone marrow mesenchymal stem cells. First, we used dual-luciferase reporter gene detection to verify coding-H19/miRNA-140-5p/Wnt1 regulatory targeting relationship. Then coding-H19 was overexpressed or silenced cells and then coding-H19, microRNA-140-5p Wnt1 expression were assessed us...

The purpose of this study was to determine the expression of long non-coding RNA (lncRNA) FTX and analyze its prognostic and biological significance in colorectal cancer (CRC). A quantitative reverse transcription PCR was performed to detect the expression of long non-coding RNA FTX in 35 pairs of colorectal cancer and corresponding noncancerous tissues. The expression of long non-coding RNA FT...

Non-coding ribonucleic acid (RNA) has without a doubt captured the interest of biomedical researchers. The ability to screen the entire human genome with high-throughput sequencing technology has greatly enhanced the identification, annotation and prediction of the functionality of non-coding RNAs. In this review, we discuss the current landscape of non-coding RNA research and quantitative anal...

micrornas constitute a group of small non-coding rnas that negatively regulate gene expression. aside from their contribution to biological and pathological pathways, altered expression of micrornas is reported in bio-fluid samples, such as serum. to employ serum's micrornas as potential biomarkers, it is crucial to develop an efficient method for microrna quantification, avoiding pre-analytica...