The determination of cholesterol is greatly important because high concentrations this biomarker are associated to heart disease. Moreover, can be used as a fuel in enzymatic cells operating under physiological conditions. Here, we present biosensor and peroxide-free biofuel cell based on the electrocatalytic oxidation NADH generated during reaction dehydrogenase (ChDH) an alternative H2O2 bios...

Sterols are ubiquitous and essential constituents of all eukaryotic cells, found either esterified and stored in droplet form (l), or unesterified and integrated into bilayers (2) . Sterols not only play a major structural role in membranes, but serve as precursors for steroid hormones and bile acids (3). The distribution of cholesterol within the cell and how it is apportioned properly are not...

Pretreatment of a serum or plasma sample with ascorbate oxidase removed interfering ascorbate and allowed the determination of cholesterol to be carried out by a current enzymatic cholesterol method available in kit form. The Cobas-Fara was programmed to carry out pretreatment of the sample with ascorbate oxidase before addition of the cholesterol colour reagent.

The renewed interest in exploring various natural habitat/environments for discovering novel microbial sources as stable cholesterol oxidase producers is on the incline due to its broad-range of clinical and industrial applications. A search was conducted to isolate cholesterol oxidase producing bacteria from samples collected from different environments. A total of 98 bacterial isolates capabl...

Article history: Received on: 15/03/2016 Revised on: 20/04/2016 Accepted on: 30/05/2016 Available online: 28/07/2016 The aim of the present research work was to optimize cholesterol oxidase production and partial sequencing of 16S rRNA gene region of Bacillus cereus strain KAVK4 by PCR. Bacillus cereus is a gram positive bacterium isolated from butter, Tamil Nadu, India and was cultured in a nu...