The addition of nitrite, the product of the reaction catalysed by nitrate reductase, to cell suspensions of the yeast Hansenula anomala caused a reversible inactivation of NADPH-dependent nitrate reductase activity. The haem- and Mo-dependent and Mo-dependent activities of nitrate reductase, determined with the non-physiological electron donors FMNH2 and reduced methyl viologen respectively, we...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 206 No. 5 655–670 www.jcb.org/cgi/doi/10.1083/jcb.201401070 JCB 655 Correspondence to Thomas L. Schwarz: [email protected] Abbreviations used in this paper: CCCP, carbonyl cyanide 3-chlorophenylhydrazone; CMV, cytomegalovirus; DIV, day in vitro; mt-KR, mitochondrial KillerRed; PD, Parkinson’s disease; ROS, reactive ox...

The phosphorylation of fructose-1,6-bisphosphatase is preceded by a transient increase in the intracellular level of cyclic AMP which activates a cyclic AMP-dependent protein kinase (Pohlig, G., and Holzer, H. (1985) J. Biol. Chem. 260, 13818-13823). Possible mechanisms by which sugars or ionophores might activate adenylate cyclase and thereby lead to an increase in cyclic AMP concentrations we...

EmrR, the repressor of the emrRAB operon of Escherichia coli, was purified to 95% homogeneity. EmrR was found to bind putative ligands of the EmrAB pump-2,4-dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, and carbonyl cyanide p-(trifluoro-methoxy)phenylhydrazone-with affinities in the micromolar range. Equilibrium dialysis experiments suggested one bound ligand per monomer of the dimer...

Avena coleoptiles excrete hydrogen ions in response to the hormone auxin. Both auxin-induced cell elongation and hydrogen ion excretion occur after only a short lag, and both are prevented by either carbonyl cyanide m-chlorophenylhydrazone or cycloheximide. These results support the idea that hydrogen ions act as a second messenger in auxin-induced cell elongation and can be the wall-loosening ...

A meiotic segregant (oliPR1) was isolated with a phenotype of multiple cross resistance and collateral sensitivity. Strain oliPR1 has increased sensitivity to ethidium bromide, dequalinium chloride, acriflavin, paromomycin and neomycin, and increased resistance to oligomycin, rutamycin, venturicidin, triethyltin bromide, antimycin, carbonylcynamide-m-chlorophenylhydrazone, tetra-N-butylammonium...

Colistin is highly promising against multidrug-resistant and extensively drug-resistant bacteria clinically. Bacteria are resistant to colistin mainly through mcr chromosome-mediated lipopolysaccharide (LPS) synthesis-related locus variation. However, the current understanding cannot fully explain resistance mechanism in -negative colistin-resistant strains. Significantly, contribution of efflu...