Monospecific antibodies have been successfully utilized in antigen detection, which is better indicator of active infection. Mycobacterium tuberculosis excretory secretory (M tb ES) antigens such as ES 31, ES 41 and ES 43 (31 kDa, 41 kDa and 43 kDa protein, respectively) have been shown to be present in Mycobacterium tuberculosis H37Ra culture filtrate and are of diagnostic interest. To study t...

introduction: the immunogenicity of crude amoebic antigen and its fractions preparedfrom entamoeba histolytica (nih:200) was evaluated in experimental animals. material and methods: forty two guinea pigs of either sex free from entamoebainfection and aged around 3 to 4 weeks were randomly divided into 5 groups. the treatedgroups consisted of 8, 10, 6, and 8 animals and 10 animals served as cont...

A diagnostic method has been developed to detect anti-Leishmania donovani immunoglobulin G (IgG) in urine by enzyme-linked immunosorbent assay (ELISA). In measuring anti-L. donovani IgG, IgA, and IgM in urine, the method performed best in the detection of IgG. The sensitivity and specificity of the assay were determined with panels of urine samples from 62 visceral leishmaniasis (VL) patients, ...

The GST antigen (called 26-28 kDa antigen) extracted and purified from Schistosoma japonicum adult worms was applied to the detection of specific antibodies in sera of infected mice and mice immunized with the above protein antigen by ELISA technique. The 26-28 kDa antigen was better than crude antigens (SEA, SWAP) when used to detect specific antibodies in sera from immunized mice. As with cru...

The aim of this study was to prepare a new formula of Leishmania major (L. major) crude antigen and evaluate its effect on immune system. For this purpose L. major promastigotes were cultured, harvested, washed, and resuspended in physiologic saline and the suspensions were dispersed in five equal batches. 0.1 ml of various doses of the cocktail antigen were injected intradermaly in three group...

Lymphocyte blastogenesis and interferon production were measured in adult human leukocyte cultures stimulated with purified or crude cytomegalovirus antigens. Leukocytes from seropositive adults underwent blastogenesis when stimulated with purified or crude Towne strain antigen, whereas neither antigen stimulated blastogenesis in cultures from seronegative donors. The concentrations of antigens...

Suzuki, T. (Children's Hospital, Buffalo, N.Y.), E. A. Gorzynski, and E. Neter. Separation by ethanol of common and somatic antigens of Enterobacteriaceae. J. Bacteriol. 88:1240-1243. 1964.-Ethanol in a concentration of 85% permits separation from crude culture supernatant fluids of the common enterobacterial antigen and the O antigen, the former being ethanol-soluble, the latter being ethanol-...

In a previous communication (Shaw, 1955), I referred to several papers concerning the value of cardiolipin antigen in complement-fixation and flocculation tests. The majority of workers have reported increased sensitivity and specificity with this antigen, which has also been recommended to obtain reproducible results in various laboratories in different countries. At that time, I compared the ...

In vitro culture of live adult worms were made in RPMI 1640 medium at a concentration of approximately 50 worms / ml in a 0 culture flask at 37 C for 24 hours and the culture supernatant was used as antigen. The E/S antigen was purified by thiol – sepharose affinity chromatography. On western blot analysis, it was demonstrated that the crude E/S antigen showed five reactive bands at 24, 29, 46,...

The performances of ELISA assays with different antigen preparations, such as Leishmania amazonensis or L. chagasi lysates and the recombinant antigens rK-39 and rK-26, were compared using sera or eluates from dried blood collected on filter paper to detect anti-Leishmania antibodies in dogs from a visceral leishmaniasis-endemic area in Brazil. Of 115 IFAT-reactive dogs at 1:40 titre, 106 (92.2...